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Endophytic bacteria have been isolated from the roots of Urtica dioica. A total of 54 endophytic bacteria were isolated from the underground parts using suitable surface sterilisation protocol. Three isolates R45a; R45b; R21a were tested for antagonism effect against Fusarium oxysporum, Colletotrichum gloeosporioides, Rhizoctonia solani, Phytophthora parasitica in dual culture method. Significant inhibitory effects on mecylial radial growth have been revealed with a percentage superior or equal to 75%. These strains were Gram-positive rods. Cultures on nutrient agar showed irregular, entirely cream coloured colonies that are strictly aerobic and capable of forming endospore. They belong probably to the genus of Bacillus spp.