Main Article Content
Intra- and inter-species genetic diversity study was carried out comparing the molecular and morphological characters of five Cola species namely Cola acuminata, Cola nitida, Cola millenii, Cola lepidota and Cola gigantea collected from five locations in Ibadan, Nigeria. Sixteen Cola accessions were analysed based on 7 quantitative characters, 12 qualitative characters and 8 Random Amplified Polymorphic DNA primers. From the quantitative characters studied, high significant difference across the location and species was recorded as leaf length had the strongest variability while the species obtained from Botany Nursery showed strongest locational effect on the vegetative leaf characters. All quantitative characters analysed were good determinants for delimiting Cola species. Cola lepidota recorded the highest variability compared to the other Cola species. Qualitative characters studied revealed that leaf surface, seed colour, and floral colour are strong characters for delimiting Cola species. Molecular analysis showed that 309 fragments were recorded, of which 28.5% were polymorphic while 8.4% were monomorphic. Genetic similarities ranged from 0.44 to 0.93 for the 16 Cola accessions with a mean value of 0.67. Deductions from the combined effect of molecular and morphological characters showed high variability for the characters analysed and a likely monophyletic relationship for the 16 Cola accessions. Molecular and morphological determinants observed in this study can help Cola breeders identify species of interest. We recommend further research on the variability of Cola species.
Sonibare AM, Soladoye MO. Esan OO. Phytochemical and Antimicrobial studies of four species of Cola Schott & Endl. (sterculiaceae). Afr. J. Tradit. Compl. Altern. Med. 2009;6(4):518-522.
Kuome, C., Sacande, M. Cola nitida (Vent) Scholt and Endl., Seed Leaflet:111.
Zou Z, Zhang J. Morphological and molecular convergences in mammalian phylogenetics Nature Comm. 2006;7:12758.
Lawrence AI, Adesoye TR. Fasola. Genetic diversity in Cola acuminata and Cola nitida using RAPD primers. Genetika. 2019;51(1):227-23.
Whittall JB, Hellquist CB, Schneider EL, Hodges SA. Cryptic species in an endangered pondweed community (Potamogeton, Potamogetonaceae) Revealed by AFLP Markers. Am J Bot. 2004;91:2022–2029.
Folkers A, Hüve K, Ammann C, Dindorf T, Kesselmeier J, Kleist E, Kuhn U, Uerlings R, Wildt J. Methanol emissions from deciduous tree species: Dependence on temperature and light intensity. Plant Biol. 2008;10(1):65–75.
Shen H, Tang Y, Muraoka H, Washitani I. Characteristics of leaf photosynthesis and simulated individual carbon budget in Primula mutans under contrasting light and temperature conditions. J Plant Res. 2008;121:191–200.
Braga MR, Adiar PM, Marabesi MA, de-Godoy JRL. Effects of elevated CO2 on the phyto alexin production of two soybean cultivars differing in the resistance to stem canker disease. Environ Exp Bot. 2006;58(1–3):85–92.
Cybulskill WJ, Peterjohn WT, Sullivan JH. The influence of ultraviolet-B radiation on tissue quality and composition of loblolly pine (Pinus taeda L.) needles. Environ Exp Bot. 2000; 44(3): 231–241.
Pierre EO, Niemenak N, Ndoumou DO. Morphological variability of Cola acuminata ((Pal. De Beauv) Schott and Endl. Germplasm in Cameroon. Pakistan J. Biol. Sci. 2006;9:398-403.
Cronquist A. Chemical Plant Taxonomy. In: G. Bendz, and J. Santesson (Eds.), Chemistry. Botanical Classification. Proceedings of the 25th Nobel Symposium, August 20-25. Academic Press, New York & London; 1981.
Saliu JA, Olabiyi AA, Adefegha SA, Oyeleye S. Comparative evaluation of antioxidative properties and effects of aqueous extracts of Cola nitida and Vitex doniana on Fe2+-Generated oxidative stress in rat testes in vitro. Int. J. Biotechnol. 2016;5(2):15-25.
Chandran K, Pandya SM. Morphological characterization of Arachis species of section, Arachis. Plant Genet. Resourc. Newslett. 2000;121:38-41.
Manzano BA, Nodals AAR, Gutierrez FZ, Mayor and Alfonso CL. Morphological and isoenzyme variability of taro (Colocasia esculenta L. Schott) germplasm in Cuba. Plant Genetic Res. Newsletter. 2001;126:31-40.
Rosso CA, Medina CI, Lobo M. Morphological characterization and agronomic evaluation of a Colombian collection of Arrachia (Arracacia xanthorrihiza Bancroft.) Plant Genetic Res. Newsletter. 2002;132: 22-29.
Mohd MA, Ibrahim H, Khalid N. Differentiation of three varieties of Zingiber officinale Rosc. by RAPD fingerprinting. Malaysian J. Sci. 2004;23:135–139.
Zhang HY, Li FS, He LL, Zhong HQ, Yang QH, He SC. Identification of Sugarcane interspecies hybrids with RAPDs. Afr. J. Biotechnol. 2008;7(8):1072-1074.
Palai SK, Rout GR. Identification and genetic variation among eight varieties of ginger by using random amplified polymorphic DNA markers Plant Biotechnol. 2007;24:417-420.
Hamrick JL, Loveless MD. The genetic structure of tropical tree populations: associations with reproductive biology. In: Bock J.H., Linhart Y.B., editors. Plant Evolutionary Ecology. 1989;131–146.
Huang H, Layne DR, Kubisiak TL. Molecular characterization of cultivated pawpaw using RAPD markers. J. American Soc. Horticul Sci. 2003;128:85–93.
Nayak S, Naik PK, Acharya LK, Pattnaik AK. Detection and evaluation of genetic variation in 17 promising cultivars of turmeric (Curcuma longa L.) using nuclear DNA content and RAPD markers Cytologia. 2006;71:49-75.