Isolation, Identification and Characterization of Phyllosticta citricarpa Causing Citrus Black Spot Disease in Benin

Goudjo Habib Toessi *

African Excellence Center on Climate Change, Biodiversity and Sustainable Agriculture (CEA-CCBAD), Félix Houphouët-Boigny University, 22 BP 582 Abidjan, Côte d'Ivoire, Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, UFR Biosciences, Félix Houphouët-Boigny University, 22 BP 582 Abidjan, Côte d'Ivoire and Laboratory of Crop Protection (LDC), National Institute of Agricultural Research of Benin (INRAB), 01 BP 884 Cotonou, Benin.

Rachidatou Sikirou

Laboratory of Crop Protection (LDC), National Institute of Agricultural Research of Benin (INRAB), 01 BP 884 Cotonou, Benin.

Elisée Georges Dadé Ler-N’ogn Amari

African Excellence Center on Climate Change, Biodiversity and Sustainable Agriculture (CEA-CCBAD), Félix Houphouët-Boigny University, 22 BP 582 Abidjan, Côte d'Ivoire and Laboratory of Biotechnology, Agriculture and Valorization of Biological Resources, UFR Biosciences, Félix Houphouët-Boigny University, 22 BP 582 Abidjan, Côte d'Ivoire

Oumarou Zoéyandé Dianda

Phytopathology Laboratory of the National Fruit and Vegetable Specialisation Centre, Burkina-Faso.

Issa Wonni

Phytopathology Laboratory of the National Fruit and Vegetable Specialisation Centre, Burkina-Faso.

Mustapha El Bouhssini

College of Agriculture and Environemental Sciences, Mohammed VI Polytechnic University (UM6P) Benguerir, Morocco.

*Author to whom correspondence should be addressed.


Abstract

Citrus black spot is a major constraint to citrus production in Benin. Identification of the pathogen causing citrus black spot disease was carried out in the laboratory on symptomatic fruits. Fruit samples were collected from orchards in 4 citrus-growing agro-ecological zones. A total of 66 representative isolates of Phyllosticta sp. were obtained from isolation. Molecular analysis using PCR of the nucleotide sequences of the ITS regions with universal primers ITS1/ITS4 and specific primers GcF1/GcR and the phylogenetic tree showed that the sequences of all isolates obtained in the different agroecological zones were identical to those of Phyllosticta citricarpa. The pathogenicity test satisfied Koch's postulates by re-isolation of Phyllosticta citricarpa from inoculated fruits. A thorough study of genetic diversity and a full understanding of the behavior of P. citricarpa will pave the way for more targeted approaches to the prevention, control and sustainable management of citrus black spot disease in Benin.

Keywords: P. citricarpa, black spot disease, citrus, PCR, phylogenetic analysis, Benin


How to Cite

Toessi, Goudjo Habib, Rachidatou Sikirou, Elisée Georges Dadé Ler-N’ogn Amari, Oumarou Zoéyandé Dianda, Issa Wonni, and Mustapha El Bouhssini. 2024. “Isolation, Identification and Characterization of Phyllosticta Citricarpa Causing Citrus Black Spot Disease in Benin”. Annual Research & Review in Biology 39 (6):41-56. https://doi.org/10.9734/arrb/2024/v39i62087.

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