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In vitro Analysis of Antimicrobial Susceptibility of Xanthomonas axonopodis pv. punicae and Comparative Study of Protein Profiling under Biotic Stress
Annual Research & Review in Biology,
Bacterial blight of pomegranate caused by X. axonopodis pv. Punicae (XAP) assumed epidemic form and resulted in economic burden on farmers. In the current study the pathogen infected samples were collected and the isolated XAP was identity and confirmed through the morphological, biochemical characterization and Pathogenicity test. Bacterium was reisolated from infected plant to prove Koch’s postulates. Efficacy of different chemicals and oils were tested by disc diffusion assay and turbidometrically. Bronopol 3000 ppm (25.6±1.6 mm) and Clove oil (18.0±0.7 mm) formed highest zone of inhibition Turbidometri showed the highest O.D. (0.908 nm) by Copper oxy chloride and Neem oil showed maximum inhibition of growth with O.D. (0.842 nm). Biotic stress (pathogen) induced protein response was studies by using SDS-PAGE method after protein extraction from XAP, healthy P. granatum L. and infected P. granatum L. The protein band pattern showed the unique band no. 2 (Mol.Wt.66000 Da) in infected P. granatum L. as compared to the banding pattern of XAP and healthy P. granatum L. The over expressed protein due to biotic stress could be useful as a marker for detection of the disease at the early stage and for control of the diseases after knowing the biochemical significance of the protein.
- bacterial blight
- Xanthomonas axonopodis pv. punicae
- in vitro efficacy
- antimicrobial activity
How to Cite
Vauterin L, Haste B, Kersters K, Swings J. Reclassification of Xanthomonas. Int. J. Syst. Bacterial. 1995;45:475-489.
Mondal KK, Sharma J. Bacterial blight: An emerging threat to pomegranate export. Ind. Farm. 2009;59:22-23.
Rangaswamy G. Pomegranate. In: bacterial plant disease in India. Asia publication house, Bombay. 1962;830.
Manjula CP, Khan ANA. Incidence of bacterial blight of pomegranate (Punica granatum L.) in Karnataka. Paper presented at the Annu. Meet. Symp. Plant disease scenario in southern India, held at Bangalore; 2002.
Antre SH, Gadhe SK, Abhang PB, Autade RH. In vitro efficacy of different chemicals, botanicals and bioagent Against Xanthomonas axonopodis pv. punicae, Int. J. Pure App. Biosci. 2016;4(3):112-118.
Kanwar ZS. A note on bacterial disease of Pomegranate (Punica granatum L.) in Haryana. Haryana J. Horti. Sci. 1976;5: 177-180.
Ryan RP, Vorhölter FJ, Potnis N, Jones JB, Van Sluys MA, Bogdanove AJ, Dow JM. Pathogenomics of Xanthomonas: Understanding bacterium-plant interactions. Nature Reviews Microbiology. 2011;9(5): 344-355.
Kishun R. Bacterial diseases of fruits. In: Chadha KL, Parek OP (Eds). Advances in Horticulture-Fruit crops, Malhotra Publishing House, New Delhi, India. 1993; 31389-1404.
Duche TR, Omoigui L, Iheukwumere CC. Variations among Xanthomonas axonopodis pv. vignicola isolates in Benue State, Nigeria. International Journal of Innovation and Scientific Research. 2015; 13(1):271-278.
Giri MS, Prasanthi Kulkarni S, Benagi VI, Hegde YR. Biochemical and molecular variability among Xanthomonas axonopodis pv. Punicae strains, the pathogen of pomegranate bacterial blight. Indian Phytopathol. 2011;64(1):1-4.
Popovic T, Josic D, Starovic M, Milovanovic P, Dolovac N, Postic D, Stankovic S. Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli. Archives of Biological Science. 2013;65:585-593.
Abdo-Hasan M, Khalil H, Debis B, Mir Ali N. Molecular characterization of Syrian races of Xanthomonas axonopodis pv. malvacearum. Journal of plant Pathology. 2008;431-439.
Kizil S, Uyar F, Sagir A. Antibacterial activities of some essential oils against plant pathogens. Asian Journal of Plant Sciences; 2005.
Pawar M, kadam M, Nirichan BK. In vitro control of bacterial blight causing organism (Xanthomonas axonopodis pv. punicae) of pomegranate. Journals of Environmental Research and Development. 2014;8(3a): 568.
Kumar Suresh, Vinutha T, Archana Singh, Rai RD et al. Training manual plantomics-emerging tools and techniques for crop Improvement; 2015.
Bio Era Life Sciences Pvt. Ltd., Pune. Teaching Kit, Total Protein Extract Kit (From Bacteria) [Brochure]. Pune, Maharashtra: Author. Catalog number BTK 130934; 2017.
Bradbury JF. Isolation and preliminary study of bacteria from plants. PANS Pest Articles & News Summaries. 1970;16(4): 632-637.
Gamangatti RB, Patil MB. Biochemical studies on Xanthomonas axonopodis pv. punicae causing bacterial blight of pomegranate. International Journal of Plant Protection. 2013;6(2):401- 404.
Bhardwaj NR, Upadhyay V, Nagar M. Biochemical characterization of Xanthomonas axonopodis pv. citri, causal agent of citrus canker; 2014.
Yenjerappa ST. Epedemiology and management of bacterial blight of pomegranate caused by Xanthomonas axonopodis pv.punicae (Hingorani and Singh) Vauterin et al. Ph. D. Thesis, Univ. Agri. Sci. Dharwad (India). 2009;147.
Meerts, Fadavi et al. In vitro control of bacterial blight causing organism (Xanthomonas axonopodis pv. Punicae) of pomegranate Journal of Environmental Research and Development. 2009;8(3a): 568.
Walker JM. Electrophoretic techniques. In Principles and Techniques of Biochemistry and Molecular Biology (6th ed.). New York: Cambridge University Press. 2005;449-485.
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