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Objectives: The present studies pursue at retrieve and draws the active phytocompounds structure of Alangium salvifolium and assessing its simulation anti-oxidant enzyme activities.
Methods: Retrieve/draws of the compounds were carried out using chem.-sketch software. The 3-D structures of the Phytocompounds were visualized based upon the UV, NMR spectral data along with their energy simulation studies. The antioxidant and enzyme simulation activity were evaluated in-silico using the ACD labs,PyRx, RASMOL,PYMOL,Aragslab and Discovery 3.1 studio.
Key Findings: Phytochemicals structure drawing of A. salvifolium resulted in the structured and recognition of four phytochemicals. The plant phytochemicals showed significant anti-oxidant enzymes activity enhancer and ROS eliminator through binding to its metal domain receptor.
Conclusion: Phytochemicals were drawing from A. salvifolium. To the best of our knowledge, among these phytochemicals, were studied anti-oxidant enzymes metals binding domain to increase the ROS scavenging activity for the foremost time from mimic with molecular docking. Moreover, study of phytochemicals simulation was for the first time from this plant. The plant revealed auspicious increase the antioxidant activities virtual screening. This gives thinking to some of its pharmacological properties and suggests additional antioxidant effects, for as a scavenger as well as anti-oxidant enzyme stimulator, which have not been reported yet.
Shinde V, Dhalwal K, Paradkar AR, Mahadik KR, Kadam SS. Evaluation of in vitro antioxidant activity of human placental extract. Pharmacol Online. 2006;3:172–9.
Uthiraselvam M, Asmathu FS, Peer MH, Babu SM, Kavitha G. Pharmacognostical studies on the medicinal plant - Alangium salvifolium (Linn. F) Wang. (Alangiaceae). Asian J. Plant Sci. Res. 2012;2(6):670-674.
Ronok Z, Laizuman N, Luthfun NM. Antinociceptive and anti-inflammatory activities of flower (Alangium salvifolium) extracts. Pak. J. Bio. Sci. 2013;16(19): 1040-1045.
Meera R, Shabina S, Devi P, Venkataraman S, Parameswari PT, Nagarajan K, Aruna A. Anti-hyperglycemic effect of aqueous and ethanolic extracts of leaf and stem bark of Alangium salvifolium (L.F.) Wang in alloxan induced diabetic rats. Inter. J. Phar. Res. Allied Sci. 2013; 2(4):28-32.
Gopinath SM. Broad spectrum anti-microbial activities and phytochemical analysis of Alangium salviifolium flower extract. Global J. Res. Med. Plants Indigen. Med. 2013;2(3):135-141.
Savithramma N, Ling RM, Ankanna S. Preliminary phytochemical screening of some important medicinal plants. Inter. J Ayur. Herbal Med. 2012;2(1):139-145.
Worthington Enzyme Manual. Worthington Biochemical Corporation; 2009.
Frank L. Prolonged survival after paraquat: Role of the lung antioxidant enzyme systems. Biochem Pharmacol. 1981;30: 2319-2324 .
Bagley A, Krall J, Lynch R. Superoxide mediates the toxicity of paraquat for Chinese hamster ovary cells. Proc Natl Acad Sci USA. 1986;83:3189-3193.
Bus JS, Gibson JE. Paraquat: Model for oxidant-initiated toxicity. Environ Health Perspect. 1984;55:37-46.
Stevens TM, Boswell GA, Adler R, Ackerman NR, Kerr JS. Induction of antioxidant enzyme activities by a phenylurea derivative, EDU. Toxicol Appl Pharmacol. 1988;96:33-42 .
Saito K. Effects of paraquat on macro-molecule synthesis in cultured pneumo-cytes. Tohoku J Exp Med. 1986;148:303-312.
Kelner M, Bagnell R. Generation of endo-genous glutathione peroxidase, manganese superoxide dismutase, and glutathione transferase activity in cells transfected with a copper-zinc superoxide dismutase expression vector. J Biol Chem. 2014;265: 10872-10875 .
St'Clair D, Oberley T, Ho YS. Over-production of Mn-superoxide dismutase modulates paraquat-mediated toxicity in mammalian cells. FEBS Lett. 2009;293: 199-203.
McKusker K, Hoidal J. Selective increase of antioxidant enzyme activity in the alveolar macrophages from cigarette smokers and smoke-exposed hamsters. Am Rev Respir Dis. 1990;141:676-682.
Toth K, Berger E, Beehler C, Repine J. Erythrocytes from cigarette smokers contain more glutathione and catalase and protect endothelial cells from hydrogen peroxide better than do erythrocytes from nonsmokers. Am Rev Respir Dis. 2015; 134:281-284.
Hay J, Shahzeidi S, Laurent G. Mechanisms of bleomycin-induced lung damage. Arch Toxicol. 1991;65:81-94.
Sausville EA, Peisach J, Horwitz SB. Effect of chelating agents and metal ions on the degradation of DNA by bleomycin. Biochemistry. 1996;17:2740-2745.
Oberley LW, Beuttner GR. The production of hydroxyl radical by bleomycin and iron (II). FEBS Lett. 1979;97:47-49.
Giri S, Chien Z, Younker W, Schiedt M. Effects of intratracheal administration of bleomycin on GSH-shuttle enzymes, catalase, lipid peroxidation and collagen content in the lungs of hamsters. Toxicol Appl Pharmacol. 2016;71:132-141.
Giri SN, Misra HP, Chandler DB, Chen Z, Younker WR. Increases in lung prolyl hydroxylase and superoxide dismutase activities during bleomycin-induced lung fibrosis in hamsters. Exp Mol Pathol. 1983; 39:317-326.
Fantone J, Phan S. (Oxygen metabolite detoxifying enzyme levels in bleomycin-induced fibrotic lungs. Free Radic Biol Med. 2008;4:399-402.
Ledwozyw A. Protective effect of liposome-entrapped superoxide dismutase and catalase on bleomycin-induced lung injury in rats. II. Phospholipids of the lung surfactant. Acta Physiol Hungar. 2012;78: 157-162.
Borzone G, Klaassen R, Vivaldi E. Bleomycin-induced lung injury in rats: Protective effect of free radical scavengers. Am Rev Respir Dis. 1992;145: A578.
Surinrut P, Shaffer J, Marsh J, Heintz NH, Mossman BT. Transfection of a human catalase gene ameliorates asbestos-induced cytotoxicity in hamster tracheal epithelial cells. Am Rev Respir Dis. 2009; 147:A205.
Kinnula VL, Everitt JI, Mangum JB, Chang LY, Crapo JD. Antioxidant defense mechanisms in cultured pleural mesothelial cells. Am J Respir Cell Mol Biol. 2013;7: 95-103.