Basil (Ocimum basilicum L.) and/or Celery (Apium graveolens L.) Leaves Aqueous Extracts Role in Opposition to Drinking Contaminated Water Induced Male Rats Urinary Stones and Renal Deteriorations
Annual Research & Review in Biology,
Aims: The current research was designed to explore the role of basil and/or celery leaves aqueous extracts against urinary stones (urolithiasis) and renal deteriorations induced by drinking water contaminated with ethylene glycol (EG) and ammonium chloride (AC) in male rats.
Place and Duration of Study: The Medical Research Center of the Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. The experiment duration was 35 days, 7 days for acclimatization followed by 28 days experimentation.
Methodology: Fifty-five adult male Sprague-Dawley rats were classified randomly into 5 groups of ten animals each, except urolithiatic control group which contained 15 rats. Rats were treated as follow: Group I: healthy control group (HCG), rats drank distilled water and received a placebo 1 ml distilled water daily by intra-gastric tube ; Group II: urolithiatic control group (UCG), rats drank distilled water containing ( 0.75% EG and 1% AC ) and the access to water was ad libitum and received 1 ml distilled water daily by intra-gastric tube.; Group III : urolithiatic rats supplemented with aqueous basil extract(U+ABE), rats drank distilled water containing ( 0.75% EG and 1% AC) and the access to water was ad libitum and supplemented with (200mg/kg body weight) aqueous basil extract daily by intra-gastric tube.; Group IV: urolithiatic rats supplemented with aqueous celery extract(U+ACE), rats drank distilled water containing ( 0.75% EG and 1% AC) and the access to water was ad libitum and supplemented with (200mg/kg body weight) aqueous celery extract daily by intra-gastric tube. ; Group V: urolithiatic rats supplemented with aqueous basil and celery extract(U+ABE+ACE) , rats drank distilled water containing ( 0.75% EG and 1% AC) and the access to water was ad libitum and supplemented with (100mg/kg body weight) aqueous basil extract and (100mg/kg body weight) aqueous celery extract daily by intra-gastric tube. At the end of the study 28 days, 24 h urine samples were collected from each individual animal and then rats were sacrificed under sodium barbiturate anesthesia. Blood samples were collected from the hepatic portal vein, serum was separated for biochemical analyses. Kidney samples were separated for tissue homogenate preparation, urinary bladder and ureters were separated and opened to collect urinary calculi.
Results: Research results documented that basil and celery aqueous extracts contain significant amount of active constituents including flavonoids and polyphenols. Drinking water contaminated with EG and AC caused significant decrease (p≤0.01) in urine volume, pH, urinary and renal magnesium levels with significant increase (p≤0.01) in urinary and renal calcium, phosphate and oxalate levels. It also caused significant increase in kidney function tests [serum creatinine, uric acid, urea, as well as cystatin C] levels .Also renal enzyme activities of [acidic phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP)] activities were significantly increased (p≤0.01) with significant decrease in renal lactate dehydrogenase (LDH) activity. Water contamination also induced renal mitochondrial dysfunction (decreased complex-1 activity), apoptosis (increased caspase-3 activity) in association with a state of renal oxidative stress [increased renal malondialdehyde (MDA) level related to decreased renal reduced glutathione (GSH)level and superoxide dismutase (SOD) activity].Inflammation [increased renal tumor necrosis factor- alpha (TNF- α) and interleukin 18 (IL-18)] with suppressed immune function [decreased serum immunoglobulin (IgG and IgM)] levels were observed in urolithiatic control group. As a result of biochemical changes resulted from EG and AM administration mineral precipitation and urinary stones formation was observed in urolithiatic control group. Oral administration of aqueous basil and /or celery extracts to urolithiatic rats improved urine analysis parameters, renal functions, renal enzymes activities and mitochondrial function. Aqueous extracts also counteracted renal oxidative stress, inflammation, suppressed immune response and urinary stones formation.
Conclusion: Research results proved that aqueous basil and/or celery extracts counteracted and ameliorated urinary stones formation and renal deteriorations associated with drinking water contaminated with EG and AC due to their content of active constituents including flavonoids and polyphenols. The most significant improvements were recorded in the group supplemented with both extracts.
- Water contamination
- ethylene glycol
- ammonium chloride
- renal deteriorations
- aqueous extracts.
How to Cite
Kumar A. and Xagoraraki I. Pharmaceuticals, personal care products and endocrine-disrupting chemicals in U.S. surface and finished drinking waters: a proposed ranking system. Science of the Total Environment.2010; 408 (23):5972-5989.
Loriaux D, Bergin SP and Patel S M. Ethylene glycol toxicity in the setting of recurrent ingestion: A Case Report and Literature Review. Cureus. 2019;11(4): 4375.
Kharicha SM, Kharicha A, Mogeritsch J, Wu M and Ludwig A .Review of ammonium chloride−water solution properties Journal of. Chemical and Engineering Data .2018; 63(9): 3170–3183.
Lonkala S. In-vitro anti-urolithiatic evaluation of tamsulosin against urolithiasis induced in wistar albino rats. Journal of Forensic Science and Toxicology. 2020; 3(1): 1011.
Kifayatullah M., Rahim H, Jan N U, Abbas A, Khan MS and Ikram M .Anti-urolithiatic effect of Pericampylus glaucus against ethylene glycol induced urolithiasis in male sprague dawley rats. Sains Malaysiana.2019; 48(5): 1075–1081.
Goyal PK, Verma SK and Sharma AK. Evaluation of antiurolithiatic effects of Parmelia perlata against calcium oxalate calculi in hyperoxaluric rats. Journal of Applied Pharmaceutical Science .2018; 8 (01): 129-135.
Li X, Wang W, Su Y, Yue Z, Bao J. Inhibitory effect of an aqueous extract of Radix Paeoniae Alba on calcium oxalate nephrolithiasis in a rat model. Renal Failure. 2017; 39(1) 120-129.
Ushakiran CH, Murthy PPA, Lakshmi P S, Mounika V, Mani J, Yothi N and Anitha P .Evaluation and antiurolithiatic activity of rapanus sativus extract by in-vivo on experimentally induced urolithiasis in rats. International journal of pharmacy and pharmaceutical analysis .2017; 1(2):71- 78
Rachmawati NA, Wasita B and Kartikasari LR. Basil leaves (Ocimum sanctum linn.) extract decreases total cholesterol levels in hypercholesterolemia sprague dawley rats model .IOP Conf. Series: Materials Science and Engineering.2019; 546 (6): 1-6.
Kooti W and Daraei NA Review of the Antioxidant Activity of Celery (Apium graveolens L).Journal of Evidence-Based Complementary & Alternative Medicine. 2017; 22(4): 1029-1034.
Fan J, Glass MA and Chandhoke PS. Impact of ammonium chloride administration on a rat ethylene glycol urolithiasis model .Scanning Microscopy.1999; 13(2-3): 299-306.
(NRC), National Research Council. Nutrient requirements of laboratory animals 4th ed. 1995.National Academy Press, Washington, DC.
Farag MFS. Utilization of basil extract as a radioprotector in male rats .Arab Journal of Nuclear Science and Applications.2013; 46(1): 274-281.
Hardani A, Afzalzadeh MR, Amirzargar A, Mansouri E, Meamar Z. Effects of aqueous extract of celery (Apium graveolens L.) leaves on spermatogenesis in healthy male rats. Avicenna Journal Phytomedicine. 2015; 5 (2): 111
Arnous A, Makrisand DP and Kefalas P. Effect of principal polyphenol components in relation to antioxidant characteristics of aged red wines. Journal of Agriculture and Food Chemistry.2001; 49: 5736.
Joyeux M, Lobestein A, Anton R, and Morier F. Comparative anti- lipoperoxidant, anti-necrotic and scavenging potencies of terpenes and bio-flavones from gingko and some flavonoids. Planta Medicine.1995; 61: 126-129.
Mariappan A, Ganapathy G and Banumathi V. Anti-urolithiatic evaluation of siddha formulation seenakara parpam against zinc disc implantation induced urolithiasis in wistar albino rats. International Journal of Advanced Research in Biological Science. 2016; 3(12):7-13.
Bhave AL, Bhatt JD, Hemavathi KG. Antiulcer effect of amlodipine and its interaction with H2blocker and proton pump inhibitor in pylorus ligated rats. Indian Journal of Pharmacology. 2006; 38(6):403-407.
Fossati P, Prencipe L, Berti G. Use of 3, 5- dichloro-2-hydroxybenzenesulfonicacid/4- aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clinical Chemistry. 1980; 26: 227-231.
Kaplan A. Urea, Kaplan A, et al. Clinical Chemistry the C.V. Mosby Co St Louis. Toronto. Princeton. 1984; 33:1257-1260.
Murray RL. Creatinine, Kaplan A, et al. Clinical Chemistry the C.V. Mosby Co, St Louis, Toronto, Princeton. 1984; 32:1261–1266.
Pergande M, Jung K. Sandwich enzyme immunoassay of cystatin C in serum with commercially available antibodies. Clinical Chemistry. 1993; 39(9):1885- 1890.
Draper HH and Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology.1990; 186:421-431.
Beutler E, Duroun O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine. 1963; 61: 882-888.
Nishikimi M, Roa NA and Yogi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Journal of Biochemical and Biophysical. Research. Communications. 1972; 46:849- 853.
Birch-Machin MA and Turnbull DM .Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues. Methods Cell Biology.2001; 65:97–117.
Dowlati Y, Herrmann N, Swardfager W, Liu H, Sham L, Reim EK and Lanctôt KL. A meta- analysis of cytokines in major depression. Biological Psychiatry. 2010; 67:446–457.
Zhong F, Xu J, Yang X, Zhang Q, Gao Z, Deng Y, Zhang L and Yu C. miR-145 eliminates lipopolysaccharides-induced inflammatory injury in human fibroblast-like synoviocyte MH7A cells. Journal of Cell Biochemistry .2018;119:10059-10066.
Lonkala S. In-Vitro Anti-urolithiatic evaluation of tamsulosin against Uurolithiasis induced in wistar albino rats. Journal Forensic Science Toxicology. 2020; 3(1): 1011.
Levesque R. SPSS programming and data management: A guide for SPSS and SAS users. 4th ed. SPSS Inc, Chicago, IL; 2007.
Sebastian MRA and George AM. Evaluation of tocolytic activity of aqueous seed extract of Syzygium Cumini on oxytocin induced preterm labor. Asian Journal of Pharmaceutical and Clinlical Research.2020; 13(5): 162-170.
Mojzer EB, Hrnˇciˇc MK, Škerge M, Knez Z, Bren U. Polyphenols: extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules. 2016;21(901):1- 38.
Taie HAA, Salama ZA, Radwan S. Potential activity of basil plant as a source of antioxidants and anticancer agents as affected by organic and bio-organic fertilization Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 119-127.
Siska S, Abdul Mun‘im , Bahtiar A and Suyatna F D. Effect of Apium graveolens Extract Administration on the Pharmacokinetics of Captopril in the Plasma of Rats .Scientia Pharmaceutia. 2018; 6:86.
Tzou, DT, Taguchi K, Chi T and Stoller ML. Animal models of urinary stone disease. International Journal of Surgery.2016; 36: 596-606.
Atmani F, Slimani Y, Mimouni M and Hacht B. Prophylaxis of calcium oxalate stones by Herniaria hirsute on experimentally induced nephrolithiasis in rats. British Journal of Urology. 2003 ;92 : 137–140.
Goyal PK, Verma SK and Sharma AK .Evaluation of antiurolithiatic effects of Parmelia perlata against calcium oxalate calculi in hyperoxaluric rats .Journal of Applied Pharmaceutical Science.2018; 8 (01): 129-135.
Salama AA,El-Kassaby MI and Hassan A. Anti-urolithiatic activity of Solanum nigrum hydroalcoholic extract in ethylene glycol-induced urolithiasis in rats .Egyptian Pharmaceutical Journal. 2019; 18:311–319.
Olayeriju OS,Crown OO , Elekofehinti OO, Akinmoladun AC, Olaleye MT and Akindahunsi AA. Effect of moonseed vine (Triclisia gilletii Staner) on ethane-1,2-diol-induced urolithiasis and its renotoxicity in Wistar albino rats. African Journal of Urology. 2020 ;26:4.
Tousson E, Keshta ATH, Hussein Y, Fekry RM, Abo-Ghaneima WK. Renal protective effect of Ginkgo biloba and l-carnitine extracts against pentylenetetrazol induced toxicity, oxidative stress, injury and proliferation alternation in epileptic rats. Annual Research & Review in Biology. 2019; 32(2):1-13.
Baheti DG and Kadam SS. Antiurolithiatic activity of a polyherbal formulation against calcium oxalate induced urolithiasis in rats Journal of Advanced Pharmacy Education & Research .2013 ;3 (1):31-41.
Sudhahar V, Veena CK, Varalakshmi P. Antiurolithic efect of lupeol and lupeol linoleate in experimental hyperoxaluria. Journal of Natural Products .2008; 71(9):1509–1512.
Taylor ER, Hurrell F, Shannon RJ, Lin TK, Hirst J, Murphy MP. Reversible glutathionylation of complex I increases mitochondrial superoxide formation. Journal of Biological Chemistry .2003;278(22):19603–19610
Zhai W, Zheng J, Yao X, Peng B, Liu M, Huang J, Wang G, Xu Y. Catechin prevents the calcium oxalate monohydrate induced renal calcium crystallization in NRK-52E cells and the ethylene glycol induced renal stone formation in rat. BMC Complementary and Alternative Medicine. 2013; 13: 228.
Keleş R, Şen A, Ertaş B, Kayalı D, Eker P, Şener TE, Doğan A, Çetinel Ş, Şener G. The effects of Urtica dioica L. ethanolic extract against urinary calculi in rats. Journal of Research in Pharmacy. 2020; 24(2): 205-217.
Divakar K, Pawar AT, Chandrasekhar SB, Dighe SB, Divakar G. Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats. Food and Chemistry Toxicology .2010; 48(4):1013–1018
Patel PK, Patel MA, Saralai MG, Gandhi TR. Antiurolithiatic effects of solanum xanthocarpum fruit extract on ethylene-glycol-induced nephrolithiasis in Rats. Journal of Young Pharmacists. 2012; 4(3): 164–170.
Aslan Z and Aksoy L. Anti-inflammatory effects of royal jelly on ethylene glycol induced renal inflammation in International Brazilian Journal of urology. 2015; 41: 1008-1013
Parikh CR, Mishra J, Thiessen-Philbrook H, Dursun B, Ma Q and Kelly C. Urinary IL-18 is an early predictive biomarker of acute kidney injury after cardiac surgery. Kidney International.2006; 70:199-203.
Faubel S, Lewis EC, Reznikov L, Ljubanovic D, Hoke TS and Somerset H. Cisplatin-induced acute renal failure is associated with an increase in the cytokines interleukin (IL) 1beta, IL-18, IL-6, and neutrophil infiltration in the kidney. Journal of Pharmacology Experimental Therapeutics.2007; 322:8-15.
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