Antimicrobial Resistance and Virulence Factors of Campylobacter coli Isolated from Chicken in Côte d’Ivoire
Annual Research & Review in Biology,
Aims: Campylobacters species are major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. The aims of this study were to analyze antimicrobial profile and virulence factors associated to Campylobacter coli isolated from chicken’s ceaca in commercial slaughter in Abidjan.
Methodology: A total of 336 chicken ceaca samples were collected from market of two municipality of Abidjan and were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility tests of the isolates were determined by disk diffusion method the presence of virulence genes was examined using simple PCR method.
Results: From these samples, 210/336 (62.50%) were positives for Campylobacter. Among the isolates, 53 strains confirmed as C. coli by using PCR detection were used for phenotypic and genotypic analysis. Of these strains, 51/53 were positive for one or more antibiotics molecules tested. The highest rate of antimicrobial resistance was found for nalidixic acid 51/53 (96.22%), tetracyclin 49/53 (92.45%) and ciprofloxacin 38/53 (71.69%). Moreover, MDR including 3, 4, 5 and 6 antibiotics families was detected in 11/53 (20.75%) of isolates. On the other hand, detection of virulence gene shows presence of cadF gene in 86.01% of isolates while 82.21% were positive for the three cdt (A, B and C) genes.
Conclusion: We reported in this study the presence of high pathogenic Campylobacter coli contamination of the studied chickens. Molecular identification of the bacteria was performed and determination of high resistance to antimicrobials of the fluoroquinolone family was revealed.
- Campylobacter coli
- virulence factors
- Côte d’Ivoire.
How to Cite
Li J, Gulbronson CJ, Bogacz M, Hendrixson DR, Thompson SA. FliW controls growth-phase expression of Campylobacter jejuni flagellar and non-flagellar proteins via the post-transcriptional regulator CsrA. Microbiol. 2018;164:1308-1319.
Platts-Mills JA, Kosek M. Update on the burden of Campylobacter in developing countries. Curr Opin Infect Dis. 2014; 27:444–450.
Young KT, Davis LM, Dirita VJ. Campylobacter jejuni: molecular biology and pathogenesis. Nat Rev Microbiol. 2007;5:665–679.
Bacon DJ, Alm RA, Burr DH, Hu L, Kopecko DJ, Ewing CP. Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infect Immunol. 2000;68: 4384–4390.
Goualié GB, Akpa EE, Kakou-N’Gazoa ES, Guessennd N, Bakayoko S, Niamké LS, Dosso M. Prevalence and antimicrobial resistance of thermophilic Campylobacter isolated from chicken in Côte d’Ivoire. Int J Microbiol. 2012;5.
Goualié BG, Ouattara HG, Akpa EE, Guessennd N, Bakayoko S, Niamké S, and Dosso M. Occurrence of multidrug resistance in Campylobacter from Ivorian poultry and analysis of bacterial response to acid shock. Food Sci. Biotechnol. 2014; 23:1185–1191.
Johannessen GS, Garofolo G, Di Seraﬁno G, Koláˇcková I, Karpíšková R, Wieczorek K, Osek J, Christensen J, Torp M, Hoorfar J. Campylobacter in chicken—Critical parameters for international, multicentre evaluation of air sampling and detection methods. Food Microbiol. 2020;90:10455. [CrossRef][PubMed]
Vinueza-Burgos C, Wautier M, Martiny D, Cisneros M, Van Damme I, De Zutter L. Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age. Poult Sci. 2017; 96:2366–2374.
Konkel ME, Gray SA, Kim BJ, Garvis SG, Yoon J. Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol. 1999;37:510–517.
Bang DD, Scheutz F, Gradel KO, Nielsen EM, Pedersen K, Enberg J, Gerner-Smidt P, Handberg K, Madsen M. Detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from different sources and cytolethal distending toxin production suggest potential diversity of pathogenic properties among isolates. Genome Lett. 2003;2:62-72.
Cover TL, Perez-Perez GI, Blaser MJ. Evaluation of cytotoxic activity in fecal filtrates from patients with Campylobacter jejuni or Campylobacter coli enteritis. FEMS Microbiol Lett. 1990;58:301–304.
Goualie GB, Akpa EE, Kakou-N’Gazoa SE, Ouattara HG, Niamke SL, Dosso M. Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire. J Infect Dev Ctries. 2019;13(8):671-677. DOI:10.3855/jidc.11355.
Reardon S. Antibiotic resistance sweeping developing world. Nature. 2014;509:141–142.
Goualie GB, Konan LMP, Karou TAG, Niamke LS. Evolution de la résistance aux antibiotiques de Campylobacter sp. isolés de poulets de chair à Abidjan, Côte d’Ivoire. J. Appl. Biosci. 2020;148:15202–15208.
Bolton FJ, Wareing DR, Skirrow MB, Hutchinson DN. Identiﬁcation and biotyping of Campylobacter. in Identiﬁcation Methods in Applied and Environmental Microbiology, G. R. Board, D. Jones, and F. A. Skinner, Eds. Blackwell Scientiﬁc Publications, Oxford, UK. 1992;151–161.
Linton, D.; Owen, R.J.; Stanley, J. Rapid identiﬁcation by PCR of the genus Campylobacter and ﬁve Campylobacter species enteropathogenic for man and animals. Res. Microbiol. 1996;147: 707–718. [CrossRef]
CASFM/EUCAST. Recommandations du CASFM / EUCAST v.1.0. Comité de l' Antibiogramme de la Société Française de Microbiologie (SFM) EUropean Committee on Antimicrobial Susceptibility Testing (EUCAST) ; 2018. Available:https://www.sfmmicrobiologie.org/2019/01/07/casfmeucast-2019/
Messad S, Hamdi TM, Bouhamed R, Ramdani BN. Isolement et Fréquence D’antibiorésistance des Campylobacter Thermotolérants Chez le Poulet de Chair Dans la Région D’Alger. Thèse., Ecole Nationale Vétérinaire, 2012;Alger. :2.
European Food Safety Agency. Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008. Part A: Campylobacter and Salmonella prevalence estimates. EFSA J. 2010;8(3):1503-1513.
Asmai R, Karraouan B, Es-Soucratti K, En-Nassiri H, Bouchrif B, Karib H, Triqui R. Prevalence and antibiotic resistance of Campylobacter coli isolated from broiler farms in the Marrakesh Safi region, Morocco, Veterinary World, 2020;13(9):1892-1897.
Salim SM, Mandal J, Parija SC. Isolation of Campylobacter from human stool samples. Indian J. Med. Microbiol. 2014; 32:35-38.
Rozynek E, Dzierzanowska-Fangrat K, SzczezepaÅ B, Wardak S, Szych J, Konieczny P, Albrecht P, Dzierzanowska D. Trends in antimicrobial susceptibility of Campylobacter isolates in Poland (2000-2007). Pol J Microbiol. 2007;58:111–115.
Ferro ID, Benetti TM, Oliveira TCRM, Abrah˜ao WM, Farah SMSS, Luciano FB, Macedo REF. Evaluation of antimicrobial resistance of Campylobacter spp. isolated from broiler carcasses. Br Poult Sci. 2015;56:66–71.
European Food Safety Authority (EFSA). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J. 2015;12:3547.
Rahimi E, Momtaz H, Ameri M, Ghasemian-Safaei H, Alikasemi M. Prevalence and antimicrobial resistance of Campylobacter species isolated from chicken carcasses during processing in Iran. Poult Sci. 2010;89:1015–1020.
Rivera FN, Bustos R, Montenegro SH, Sandoval M, Castillo N, Fern´andez J, Maturana R, Delgado R, Contreras S, Chavez ND, Chavez N, Quevedo L. Genotyping and antibacterial resistance of Campylobacter spp strains isolated in children and in free range poultry. Rev Chil infectologıa. 2011;28:555–562. [Article in Spanish].
Alfredson DA, Korolik V. Antibiotic resistance and resistance mechanisms in Campylobacter jejuni and Campylobacter coli. FEMS Microbiol Letters. 2007; 277(2):123–132.
Goualie GB, Bakayoko S, Coulibaly KJ. Practices of biosecurity measures and their consequences on poultry farms in Abidjan district. Food and Environment Safety. 2020;19(1):33-39.
Anja K, Pogačar MŠ, Raspor P, Abram M, Možina SS, Vučković D. Virulence genes and cytokine profile in systemic murine Campylobacter coli infection. Virulence. 2015;6(6):581-590. DOI: 10.1080/21505594.2015.1042642
Khoshbakht R, Tabatabaei M, Hosseinzadeh S, Shekarforoush SS, Aski SH. Distribution of nine virulence-associated genes in Campylobacter jejuni and C. coli isolated from broiler feces in Shiraz, southern Iran. Foodborne Pathog Dis. 2013;10(9):764-70. PMID:23789768; Available:http://dx.doi.org/10.1089/fpd.2013.1489
Lee J, Jeong J, Lee H, Ha J, Kim S, Choi Y, Lee S. Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry. Int. J. Environ. Res. Public Health. 2017;14(11):1400. DOI:10.3390/ijerph14111400
Datta S, Niwa H, Itoh K. Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. J Med Microbiol. 2003;53:345– 348.
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