Antibiotic Sensitivity Patterns and Plasmid Profile of Bacteria Isolated from Some Swimming Pools in Akure, Nigeria

Main Article Content

A. K. Onifade
B. E. Francis
E. A. Aiyenuro

Abstract

Aims: To evaluate the antibiotic sensitivity profile of bacteria isolated from swimming pools in Akure, Nigeria.

Place and Duration of Study: Department of Microbiology, The Federal University of Technology, Akure Ondo state Nigeria between May and July, 2018.

Methodology: Water samples were collected in the morning and evening periods including weekends, from ten (10) swimming pools in Akure, Ondo State, Nigeria. The temperature and the pH of the water samples were measured and recorded at the time of collection. The types and loads of bacteria at different times of each day were determined for each of the swimming pools. Characterization and Identification of the various bacterial isolates were based on Gram-staining techniques and biochemical tests. Antibacterial susceptibility profile of the isolates was evaluated using standard methods. Escherichia coli and Staphylococcus aureus being resistant to multiple antibiotics were subjected to plasmid analysis.

Results: Bacteria isolated include; E. coli which had the highest occurrence rate (25%), S. aureus (18.75%), Shigella flexneri (14.50%), Klebsiella pneumoniae (14.50%), Proteus mirabilis (9.37%), Citrobacter freundii (6.25%) Pseudomonas aeruginosa (6.25%) Enterobacter faecalis (3.13%) and Salmonella bongori (3.13%). Ciprofloxacin, pefloxacin and tarivid recorded remarkable zones of inhibition against the isolates. The isolates were notably resistant towards chloramphenicol and septrin. The isolates were further examined for the presence of conjugative plasmids. The results showed that their resistance was chromosomal mediated.

Conclusion: Despite the fact that these pools meet the World Health Organization minimum requirement, Microbiological examination of the swimming pools revealed that most of them are contaminated with various pathogenic microorganisms which are potentially harmful.

Keywords:
Plasmid, resistance, antimicrobials, bacteria, antibiotics.

Article Details

How to Cite
Onifade, A. K., Francis, B. E., & Aiyenuro, E. A. (2020). Antibiotic Sensitivity Patterns and Plasmid Profile of Bacteria Isolated from Some Swimming Pools in Akure, Nigeria. Annual Research & Review in Biology, 35(11), 118-125. https://doi.org/10.9734/arrb/2020/v35i1130305
Section
Original Research Article

References

Ajadi FA, Bakare MK, Oyedeji O. Assessment of the physicochemical and microbiological qualities of swimming pools in selected hotels in Osogbo metropolis, Southwestern Nigeria. Ife Journal of Science. 2016;8(4):831-843.

Esinulo AC, Ogbuagu DH. Quality assessment of selected public swimming pools in Owerri Metropolis, Nigeria. International Journal Innovative Environ-mental Studies Research. 2016;1(1):1-6.

Yongsi HBN. Suffering for water, suffering from water: Access to drinking-water and associated health risks in Cameroon. Journal of Health, Population and Nutrition. 2010;28(5):424-435.

Saberianpour S, Momtaz H, Ghanbari F, Mahmodi F. Assessment of bacterial and fungal contamination in public swimming pools in Shahrekord, Iran. Journal of Tropical DIseases. 2015;4(2):1-4.

Anyim C, Nwuzo AC, Nworie O, Oko MO, Aga MV. Evaluation of water quality used for drinking and swimming purposes in Ishiagu community, Ebonyi State. Journal of Chemical, Biological and Physical Sciences. 2013;3(1):581-587.

Soyege AO, Ajayi AO, Ngwenya E, Basson AK. Okoh AI. Vancomycin and oxacillin co-resistance of commensal staphylococci. Jundishapur Journal of Microbiology. 2014;7(4):12-14.

Onifade AK, Agunloye OO. Antibacterial assessment of crude and fractionated extracts of Vernonia amygdalina leaf against multiple antibiotic resistant bacteria of wound infection. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2019;7(2):1-12.

Zorasi CB, Richard O, Nonso A. Analysis of bacteriological quality of some selected swimming pools in Port Harcourt Metropolis. Research Journal of Pure Science and Technology. 2017;1(1):1-6.

Ekopai JM, Musisi NL, Onyuth H, Namara BG, Sente C. Determination of bacterial quality of water in randomly selected swimming pools in Kampala City, Uganda. New Journal of Science. 2017;1-7.

Ayandele AA, Adebayo EA, Oladipo EK. Assessment of microbiological quality of outdoor swimming pools in Ilorin, Kwara State. Journal of Environmental Science, Toxicology and Food Technology. 2015;9(8):25-30.

Onifade AK, Aiyenuro EA. Antimicrobial susceptibility profile of microorganisms isolated from the intestine and body parts of the African Giant Land Snail (Achatina achatina) sold in Akure, Nigeria. Journal of Advances in Microbiology. 2018;9(4):1-8.

Alaofin S, Onifade AK. Anti-bacterial activity of Tithonia diversifolia, Secamore afzelli and Jaundea pinnata against plasmid-bearing multiple antibiotics resistance bacteria from different water sources in Akure, Nigeria. Advances in Bioscience and Bioengineering. 2019;7(2): 22-26.

Onajobi IB, Okerentugba PO, Okonko IO. Physicochemical and bacteriological studies of selected swimming pool water in Ilorin Metropolis, Kwara state, Nigeria. Stem Cell. 2013;4(4):1-12.

Joyce ME, Nathan LM, Howard O, Benigna GN, Celsus S. Determination of bacterial quality of water in randomly selected swimming pools in Kampala City, Uganda. New Journal of Science. 2017;4(2):1- 7.

Tsaku PA, Ehinmidu JO, Mohammed SA. Antibiotic susceptibility and plasmid profile of Escherichia coli from door handles in two tertiary institutions in Nasarawa State, Nigeria. Journal of Advances in Microbiology. 2017;3(3):1-13.

Dick, Asitona A, Abu GO, Ibe SN. Antibiotic sensitivity and plasmid profiles of bacteria isolated from water sources in Oproama community in the Niger Delta. Biokemistri. 2015;27(1):14–21.