Sperm Lipid Peroxidation and Antioxidants Activities as Predictors of Pregnancy Outcomes after Density Gradient Centrifugation in Medical Assistance Procreation
Annual Research & Review in Biology,
Aims: The purpose of this study was to assess the Density Gradient Centrifugation (DGC) effectiveness in spermatozoa selection by analyzing the oxidative stress profile and in pregnancy prediction outcomes of In Vitro Fertilization (IVF).
Study Design: This was a prospective study.
Place and Duration of Study: Department of Biology, laboratory of Physiopathology Molecular Genetic and Biotechnology. Ain Chock Faculty of Sciences, Hassan II University, Casablanca. And Anfa Fertility Center Private Clinic of in vitro fertilization and Endoscopic Surgery, Casablanca.
Methodology: We included 101 men patients subdivided into three groups. Group A with normal sperm parameters and a positive pregnancy; Group B with normal sperm parameters and a negative pregnancy; Group C with abnormal sperm parameters and a negative pregnancy. After DGC, lipid peroxidation (MDA) level, as well as the antioxidant enzyme activities of superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT), were evaluated.
Results: Patients with normal and abnormal sperm parameters showed that MDA level, and antioxidant enzymes activities increased significantly from the pellet, to 80 %, and from 80% to 40% fractions. In addition, lipid peroxidation and enzymes activities levels were significantly lower in patients with positive pregnancy than in patients with negative pregnancy and especially those with abnormal spermatic parameters.
Conclusion: The DGC would select sperm not only motile and viable but with less oxidative stress. Moreover, the rate of lipid peroxidation and antioxidant enzyme activities could differentiate between patients with normal and abnormal spermatic parameters and between patients with positive and negative pregnancy outcome.
- lipid peroxidation
- antioxidant enzymes
How to Cite
Berkowitz JM. Mummy was a fetus: Motherhood and fetal ovarian transplantation. J Med Ethics. 1995;21:298–304.
Thonneau P, Marchand S, Tallec A, Ferial ML, Ducot B, Lansac J, et al. Incidence and main causes of infertility in a resident population (1850000) of three french regions (1988-1989). Hum Reprod. 1991;6:811–6.
Chapuis A, Gala A, Ferrières-Hoa A, Mullet T, Bringer-Deutsch S, Vintejoux E, et al. Sperm quality and paternal age: Effect on blastocyst formation and pregnancy rates. Basic Clin Androl. 2017;27.
Bungum M. Sperm DNA Integrity Assessment: A New Tool in Diagnosis and Treatment of Fertility. Obstet Gynecol Int. 2012:1–6.
Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G. Sperm DNA fragmentation: Threshold value in male fertility. Hum Reprod. 2005;20:3446–51.
Gibbons WE. Unexplained infertility Unexplained infertility. Fertil Steril. 2014;39:19–23.
John Aitken R, De Iuliis GN, Drevet JR. Role of oxidative stress in the etiology of male infertility and the potential therapeutic value of antioxidants. Oxid. Antioxidants, Impact Oxidative Status Male Reprod. Elsevier. 2018;91–100.
Xing J. AB042.Oxidative stress and male infertility. Transl Androl Urol. 2018;7:AB042–AB042.
Saalu LC. The incriminating role of reactive oxygen species in idiopathic male infertility: An evidence based evaluation. Pakistan J Biol Sci. 2010;13:413–22.
Tvrdá E, Kňažická Z, Bárdos L, Massányi P, Lukáč N. Impact of oxidative stress on male fertility - A review. Acta Vet Hung 2011;59:465–84.
Aitken R. Molecular mechanisms regulating human sperm function. Mol Hum Reprod. 1997;3:169–73.
Aitken RJ. The Amoroso Lecture The human spermatozoon - a cell in crisis? Reproduction. 1999;115:1–7.
Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;1.
Sánchez R, Sepúlveda C, Risopatrón J, Villegas J, Giojalas LC. Human sperm chemotaxis depends on critical levels of reactive oxygen species. Fertil Steril. 2010;93:150–3.
Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: An overview of the literature. Reprod Biomed Online. 2004;8:616–27.
Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: Physiology and pathology. Int J Androl. 1997;20:61–9.
Fraga CG, Motchnik PA, Shigenaga MK, Helbock HJ, Jacob RA, Ames BN. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc Natl Acad SciUSA. 1991;88:11003–6.
Zini A, Finelli A, Phang D, Jarvi K. Influence of semen processing technique on human sperm DNA integrity. Urology. 2000;56:1081–4.
Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod. 2000;15:1338–44.
De Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: A balancing act between beneficial and detrimental effects. Hum. Reprod. 1995;10:15–21.
Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril. 1992;57:409–16.
Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: Potential cause for DNA fragmentation in human spermatozoa. Hum Reprod. 1998;13:896–900.
Pasqualotto FF, Sundaram A, Sharma RK, Borges Jr E, Pasqualotto EB, Agarwal A. Semen quality and oxidative stress scores in fertile and infertile patients with varicocele. Fertility and Sterility. 2008; 89(3):602-607.
Dorostghoal M, Kazeminejad SR, Shahbazian N, Pourmehdi M, Jabbari A. Oxidative stress status and sperm DNA fragmentation in fertile and infertile men. Andrologia. 2017;49(10):12762.
Krausz C, Mills C, Rogers S, Tan SL, Aitken RJ. Stimulation of oxidant generation by human sperm suspensions using phorbol esters and formyl peptides: Relationships with motility and fertilization in vitro. Fertil Steril. 1994;62:599–605.
Sukcharoen N, Keith J, Irvine DS, Aitken RJ. Predicting the fertilizing potential of human sperm suspensions in vitro: Importance of sperm morphology and leukocyte contamination. Fertil Steril. 1995;63:1293–300.
Sukcharoen N, Keith J, Irvine DS, Aitken RJ. Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: The effect of the delay between testing and IVF. Hum Reprod. 1996;11:1030–4.
Centola GM, Herko R, Andolina E, Weisensel S. Comparison of sperm separation methods: Effect on recovery, motility motion parameters and hyperactivation. Fertil Steril. 1998;70:1173–5.
Mathieu C, Lucas H. Tests de selection des spermatozoides avant assistance medicale a la procreation. Andrologie. 1999;9:286–94.
FORD WCL. The role of oxygen free radicals in the pathology of human spermatozoa: Implications of IVF. Clin IVF Forum Curr Views Assist Reprod Manchester Univ. 1990:123–39.
Mortimer D. Sperm preparation techniques and iatrogenic failures of in-vitro fertilization. Hum Reprod. 1991;6:173–6.
Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, van Zyl JA, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46:1118–23.
Aboulmaouahib S, Madkour A, Kaarouch I, Saadani B, Sefrioui O, Louanjli N, et al. Effect of semen preparation technique and its incubation on sperm quality in the Moroccan population. Andrologia. 2017;49.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–54.
Samokyszyn VM, Marnett LJ. Inhibition of microsomal lipid peroxidation by 13-cis-retinoic acid. Methods Enzymol. 1990; 190:281–8.
Paoletti F, Aldinucci D, Mocali A, Caparrini A. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem. 1986;154:536–41.
Di Ilio C, Polidoro G, Arduini A, Muccini A, Federici G. Glutathione peroxidase, glutathione reductase, glutathione S-transferase, and γ-glutamyltranspeptidase activities in the human early pregnancy placenta. Biochem Med. 1983;29:143–8.
Aebi H. Catalase in Vitro. Methods Enzymol. 1984;105:121–6.
Allamaneni SSR, Agrawal A, Rama S, Ranganathan P, Sharma RK. Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa. Asian J Androl. 2005;7:86–92.
Takeshima T, Yumura Y, Kuroda S, Kawahara T, Uemura H, Iwasaki A. Effect of density gradient centrifugation on reactive oxygen species in human semen. Syst Biol Reprod Med. 2017;63:192–8.
AITKEN RJ, CLARKSON JS. Significance of Reactive Oxygen Species and Antioxidants in Defining the Efficacy of Sperm Preparation Techniques. J Androl. 1988;9:367–76.
Enciso M, Iglesias M, Galán I, Sarasa J, Gosálvez A, Gosálvez J. The ability of sperm selection techniques to remove single-or double-strand DNA damage. Asian J Androl. 2011;13:764–8.
Yumura Y, Iwasaki A, Saito K, Ogawa T, Hirokawa M. Effect of reactive oxygen species in semen on the pregnancy of infertile couples: Original Article: Clinical Investigation. Int J Urol. 2009;16:202–7.
Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl. 2014;16:31–8.
Pasqualotto FF, Sharma RK, Kobayashi H, Nelson DR, Thomas AJ, Agarwal A. Oxidative stress in normospermic men undergoing infertility evaluation. J Androl. 2001;22:316–22.
Pasqualotto FF, Sharma RK, Pasqualotto EB, Agarwal A. Poor semen quality and ROS-TAC scores in patients with idiopathic infertility. Urol Int. 2008;81:263–70.
Haiba F, Kerboua K, Ait Hami N, et al. Stress oxydatif et infertilité masculine: premiers résultats de l’expérience algérienne pilote à l’HMRUO/2 RM. Rev Médicale l’HMRUO. 2014:1–2014.
Deepinder F, Cocuzza M, Agarwal A. Should seminal oxidative stress measurement be offered routinely to men presenting for infertility evaluation? Endocr Pract. 2008;14:484–91.
Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 2018;16:35–43.
Dandekar SP, Nadkarni GD, Kulkarni VS, Punekar S. Lipid peroxidation and antioxidant enzymes in male infertility. J Postgrad Med. 2002;48:186–9.
Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: An update. Am J Reprod Immunol. 2008;59:2–11.
Tremellen K, King B V, Li HCX, Lu YCW. Oxidative Stress and Male Infertility : A Clinical Perspective Oxidative stress and male infertility — a clinical perspective Antioxidant Therapy for the Enhancement of Male Reproductive Health : A Critical Review of the in human seminal plasma : Determin. Hum Reprod Update. 2018; 25:2018.
Aitken RJ, Finnie JM, Muscio L, Whiting S, Connaughton HS, Kuczera L, et al. Potential importance of transition metals in the induction of DNA damage by sperm preparation media. Hum Reprod. 2014; 29:2136–47.
Potts RJ, Mjefferies T, Notarianni LJ. Antioxidant capacity of the epididymis. Hum Reprod. 1999;14:2513–6.
Alkan I, Şimşek F, Haklar G, Kervancioǧlu E, Özveri H, Yalçin S, et al. Reactive oxygen species production by the spermatozoa of patients with idiopathic infertility: Relationship to seminal plasma antioxidants. J Urol. 1997;157:140–3.
Siciliano L, Tarantino P, Longobardi F, Rago V, De Stefano C, Carpino A. Impaired seminal antioxidant capacity in human semen with hyperviscosity or oligoasthenozoospermia. J Androl. 2001;22:798–803.
Storey B. Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Mol Hum Reprod. 1997;3:203–13.
Murawski M, Saczko J, Marcinkowska A, Chwiłkowska A, Gryboś M, Banaś T. Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men. Folia Histochem. Cytobiol. 2007;45:123–6.
Halliwell B. The antioxidant paradox. Lancet. 2000;355:1179–80.
Aitken RJ, Paterson M, Fisher H, Buckingham DW, van Duin M. Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function. J Cell Sci. 1995;108:2017–25.
De Lamirande E, Tsai C, Harakat A, Gagnon C. Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates. J Androl. 1998;19:585–94.
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