In-vitro Testing of Antioxidant, Anti-Parasite Activities, Cytotoxicity, and Chemical Evaluation of Abutilon Pannosum and Cassia Occidentalis Ethanolic Extracts
Annual Research & Review in Biology,
Aims: The aim of this study to detect Anti-giardia, antioxidant activities, cytotoxicity and evaluated the chemical constituent of ethanolic extracts of Abutilon pannosum and Cassia occidentalis.
Study Design: Various standard methods were used to detect of bioactivity for ethanolic extracts of plants used in this study.
Place and Duration of Study: This study was conducted in the laboratories of microbiology and parasitology and chemistry, the International University of Africa, Khartoum, Sudan, during May 2019.
Methodology: The ethanolic extract of Abutilon pannosum and Cassia occidentalis was used as an anti-giardia and anti-oxidant in-vitro, and toxicity tests were performed using brine shrimp and MTT assay. Also, the compounds of the plants used were detected by the GCMS apparatus.
Results: The ethanolic extracts of Abutilon pannosum showed high Anti-giardia activity (79%) in concentration (500 ppm) after 72 hours, whereas the activity of Cassia occidentalis extract showed (61%). The highest antioxidant activity of ethanolic extract of Cassia occidentalis was (68.7%), while it was weak in Abutilon pannosum ethanolic extract (45%) by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results of cytotoxicity revealed that the ethanolic extracts are highly toxic to brine shrimp, but are not toxic to normal cell line (MTT). Chromatographic analysis using gas chromatography-mass spectroscopy (GCMS) showed good separation of compounds. GCMS detected 22 and14 important compounds in Abutilon pannosum and Cassia occidentalis extracts respectively. The common compound in both plant extracts is n-Hexadecanoic acid. This acid was reported as an antioxidant.
Conclusion: This study revealed that the biological activities of Abutilon pannosum extracts showed high activities of Anti-giardia and antioxidants. Non-cytotoxic in the normal cell line was shown. Cassia occidentalis showed high activity of Anti-giardia and weak activity antioxidant.
- abutilon pannosum
- cassia occidentalis
How to Cite
Karar M, Rezk A, Abdalla T, Ebrahim A, Ullrich S, Kuhnert N. Antimicrobial, antiparasitic and antioxidant activities of medicinal plants from Sudan. Journal of Alternative and Complementary Medicine Health. 2017;2:555597.
Sen A, Batra A. Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. International Journal of Current Pharmaceutical Research. 2012;4(2):67-73.
Aadesariya MK, Ram VR, Dave PN. Extraction, Isolation and Identification of Useful Phyto Constituents from Dichloromethane Leave Extract of Abutilon Pannosum and Grewia Tenax Using Q-TOF LC/MS. International Journal of Advanced Research in Chemical Science. 2017;4(10):1 - 14.
Arya V, Yadav S, Kumar S, Yadav J. Antimicrobial activity of Cassia occidentalis L (leaf) against various human pathogenic microbes. Life Sciences and Medicine Research. 2010;9:e12.
Cedillo‐Rivera R, Chávez B, González‐Robles A, Tapia A, Yépez‐Mulia L. In vitro effect of nitazoxanide against Entamoeba histolytica, Giardia intestinalis and Trichomonas vaginalis trophozoites. Journal of Eukaryotic Microbiology. 2002;49:201-208.
Arguello-García R, Cruz-Soto M, Romero-Montoya L, Ortega-Pierres G. Variability and variation in drug susceptibility among Giardia duodenalis isolates and clones exposed to 5-nitroimidazoles and benzimidazoles in vitro. Journal of Antimicrobial Chemotherapy. 2004;54:711-721.
Eltayeb OM, Jobara MI, Mohamed MA, Elshikh AA, Masaad AM. In vitro evaluation of antimicrobial, antioxidant activities and phytochemical screening of Quercus infectoria (galls) used in traditional medicine. Current Research in Microbiology and Biotechnology. 2015;3(2):595-599.
Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols DJ, Mclaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta medica. 1982;45:31-34.
Oladimeji H, Nia R, Essien E. In-vitro anti-microbial and brine-shrimp lethality potential of the leaves and stem of Calotropis procera (ait). African Journal of Biomedical Research. 2006;9(3):205 - 211.
Patel S, Gheewala N, Suthar A, Shah A. In-vitro cytotoxicity activity of Solanum nigrum extract against Hela cell line and Vero cell line. International journal of pharmacy and pharmaceutical sciences. 2009;1:38-46.
Kabbashi AS, Osman EE, Garbi MI, Ahmed IF, Saleh MS, Badri AM, Elshikh A A, Abuzeid N, Koko WS, Dahab MM. In vitro Antiprotozoal activities and cytotoxicity of selected Sudanese medicinal plants. International Journal of Biomedical Engineering and Clinical Science. 2017;3(2):6-13
Osman OAA, Abadi RSM, Ayoub SMH. Chemical constituents and antioxidant activity of some Sudanese medicinal plants. Journal of Pharmacognosy and Phytochemistry. 2018;7(6):1751-1755.
Abstract View: 140 times
PDF Download: 56 times