Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles
Annual Research & Review in Biology,
Page 61-74
DOI:
10.9734/arrb/2022/v37i1230558
Abstract
Background: The process of preimplantation embryo development in vitro represents a key phase during in vitro fertilization (IVF) cycles. It involves several regulatory signaling pathways as well as an optimized in vitro culture system. The resulting embryo quality helps to determine embryo competence before implantation and pregnancy outcomes. Reactive oxygen species (ROS) are known to play a major role in influencing the process of embryo development. Their role can be reflected in the regulation of signaling pathways as second messengers or in the irreversible cell alterations due to oxidative stress following an excess of ROS levels.
Methods: In this study, we investigated the association between morphological embryo quality (fertilization, cleavage quality, and fragmentation levels) and lipid peroxidation levels (Malondialdehyde) in embryo culture media. After intracytoplasmic sperm injection (ICSI), a total of 103 oocytes were evaluated on day 1 and day 3 of their development, and their corresponding culture media were analyzed by estimating MDA levels using thiobarbituric acid.
Results: The results showed no significant association between MDA levels in culture media and fertilization rate (p=0.3), sperm quality (p=0.99; p= 0.17; p=0.46; p=0.30; p=0.65; p=0.44; p=0.09; p=0.15; p=0.56), embryo fragmentation levels (p=0.79; p=0.40), AMH levels (p=0.31; p=0.36) and female age (p=0.60; p=0.34). However, we revealed a significant association between cleavage quality and MDA levels in the embryo environment (p=0.03).
Conclusion: We conclude that oxidative stress in IVF culture media might be mainly associated with delayed embryonic development.
Keywords:
- Embryo development
- reactive oxygen species
- malondialdehyde
- oxidative stress
How to Cite
Jamil, M., Debbarh, H., Kabit, A., Ennaji, M., Zarqaoui, M., Senhaji, W., Louanjli, N., & Cadi, R. (2022). Lipid Peroxidation Status in Embryo Culture Media: The Impact on Fertilization and Embryo Quality during IVF Cycles. Annual Research & Review in Biology, 37(12), 61-74. https://doi.org/10.9734/arrb/2022/v37i1230558
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Available:http://dx.doi.org/10.1016/j.cub.2014.03.034
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Available:https://doi.org/10.1016/j.celrep.2017.12.042
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Guérin P, El Mouatassim S, Ménézo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update. 2001;7(2):175–89.
Lijie X, Ullah O, Haixing L, Ali I, Li Z, Fang NZ. Pioglitazone mediated reduction in oxidative stress and alteration in level of PPARγ, Nrf2 and antioxidant enzyme genes in mouse preimplantation embryo during maternal to zygotic transition. Pak J Zool. 2019;51(3):1085–92.
Hensley K, Robinson KA, Gabbita SP, Salsman S, Floyd RA. Reactive oxygen species, cell signaling, and cell injury. Free Radic Biol Med. 2000;28(10):1456–62.
Nixon VL, McDougall A, Jones KT. Ca2+ oscillations and the cell cycle at fertilisation of mammalian and ascidian eggs. Biol Cell. 2000;92(3–4):187–96.
Jones DP. Radical-free biology of oxidative stress. Am J Physiol - Cell Physiol. 2008; 295(4).
Ahmadi H, Fathi F, Moeini A, Amidi F, Sobhani A. Evaluation of prooxidant-antioxidant balance in in vitro fertilization-conceived mice. Clin Exp Reprod Med. 2018;45(2):82–7.
Combelles CMH, Gupta S, Agarwal A. Could oxidative stress influence the in-vitro maturation of oocytes? Reprod Biomed Online [Internet]. 2009;18(6):864–80.
Available:http://dx.doi.org/10.1016/S1472-6483(10)60038-7
Lee R, Margaritis M, M. Channon K, Antoniades C. Evaluating oxidative stress in human cardiovascular disease: Methodological aspects and considerations. Curr Med Chem. 2012; 19(16):2504–20.
Truong TT, Soh YM, Gardner DK. Antioxidants improve mouse preimplantation embryo development and viability. Hum Reprod. 2016;31(7): 1445–54.
Truong T, Gardner DK. Antioxidants improve IVF outcome and subsequent embryo development in the mouse. Hum Reprod. 2017;32(12):2404–13.
Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, et al. Characterization of subsets of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility. Hum Reprod. 2001;16(9):1912–21.
Borowiecka M, Wojsiat J, Polac I, Radwan M, Radwan P, Zbikowska HM. Oxidative stress markers in follicular fluid of women undergoing in vitro fertilization and embryo transfer. Syst Biol Reprod Med. 2012;58(6):301–5.
Ahmed W, Lingner J. Impact of oxidative stress on telomere biology. Differentiation. 2018;99:21–7.
Lan KC, Lin YC, Chang YC, Lin HJ, Tsai YR, Kang HY. Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development. J Assist Reprod Genet. 2019;36(2):325–34.
J. Premkumar B, Agarwal A. Female infertility and assisted reproduction: Impact of oxidative stress- An update. Curr Womens Health Rev. 2012;8(3):183–207.
Kim SG, Kim YY, Park JY, Kwak SJ, Yoo CS, Park IH, et al. Early fragment removal on in vitro fertilization day 2 significantly improves the subsequent development and clinical outcomes of fragmented human embryos. Clin Exp Reprod Med. 2018; 45(3):122–8.
Soto-Heras S, Paramio MT. Impact of oxidative stress on oocyte competence for in vitro embryo production programs. Res Vet Sci [Internet]. 2020;132:342–50.
Available:https://doi.org/10.1016/j.rvsc.2020.07.013
Ribas-Maynou J, Yeste M, Salas-Huetos A. The relationship between sperm oxidative stress alterations and IVF/ICSI outcomes: A systematic review from nonhuman mammals. Biology (Basel). 2020;9(7):1–18.
Desjardins P, Hansen JB, Allen M. Microvolume protein concentration determination using the NanoDrop 2000c Spectrophotometer. J Vis Exp. 2010; (33):1–4.
Jamil M, Debbarh H, Aboulmaouahib S, Aniq Filali O, Mounaji K, Zarqaoui M, et al. Reactive oxygen species in reproduction: Harmful, essential or both? Zygote; 2020.
Chen Q, Vazquez EJ, Moghaddas S, Hoppel CL, Lesnefsky EJ. Production of reactive oxygen species by mitochondria: Central role of complex III. J Biol Chem. 2003;278(38):36027–31.
Luberda Z. The role of glutathione in mammalian gametes. Reprod Biol. 2005; 5(1):5–17.
Camello-Almaraz C, Gomez-Pinilla PJ, Pozo MJ, Camello PJ. Mitochondrial reactive oxygen species and Ca2+ signaling. Am J Physiol - Cell Physiol. 2006;291(5):1082–8.
Camello-Almaraz MC, Pozo MJ, Murphy MP, Camello PJ. Mitochondrial production of oxidants is necessary for physiological calcium oscillations. J Cell Physiol. 2006;206(2):487–94.
Feissner RF, Skalska J, Gaum WE, Sheu SS. Crosstalk signaling between mitochondrial Ca2+ and ROS. Front Biosci. 2009;14(4):1197–218.
Takahashi T, Takahashi E, Igarashi H, Tezuka N, Kurachi H. Impact of oxidative stress in aged mouse oocytes on calcium oscillations at fertilization. Mol Reprod Dev. 2003;66(2):143–52.
Jana SK, K NB, Chattopadhyay R, Chakravarty B, Chaudhury K. Upper control limit of reactive oxygen species in follicular fluid beyond which viable embryo formation is not favorable. Reprod Toxicol [Internet]. 2010;29(4):447–51.
Available:http://dx.doi.org/10.1016/j.reprotox.2010.04.002
Bedaiwy MA, Falcone T, Mohamed MS, Aleem AAN, Sharma RK, Worley SE, et al. Differential growth of human embryos in vitro: Role of reactive oxygen species. Fertil Steril. 2004;82(3):593–600.
Martín-Romero FJ, Miguel-Lasobras EM, Domínguez-Arroyo JA, Gonzélez-Carrera E, Álvarez IS. Contribution of culture media to oxidative stress and its effect on human oocytes. Reprod Biomed Online [Internet]. 2008;17(5):652–61.
Available:http://dx.doi.org/10.1016/S1472-6483(10)60312-4
Shih YF, Lee TH, Liu CH, Tsao HM, Huang CC, Lee MS. Effects of reactive oxygen species levels in prepared culture media on embryo development: A comparison of two media. Taiwan J Obstet Gynecol [Internet]. 2014;53(4):504–8.
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