Multiple Resistance in Clinical Isolates of Escherichia coli and Plasmid-mediated Quinolone Resistance Genes
Annual Research & Review in Biology,
Page 1-11
DOI:
10.9734/arrb/2023/v38i230568
Abstract
Quinolone antibiotics have been commonly used to treat cases of multiple antibiotic resistance. Unfortunately, quinolone antibiotics have so much been resisted by infectious bacterial agents. This study aimed to evaluate the susceptibility of some clinical isolates of E. coli to some commonly used quinolone antibiotics and the determination of the plasmid-encoded quinolone resistance genes.
Our results showed the plasmid quinolone-resistance genes in the following prevalence: qnr genes: qnr S (71.4 %); qnr B (15.4 %); qnr S and B (12.1 %); aac (6) lb-cr (4 %); Efflux genes: oqxA (7.7 %); oqxB (25.3 %); qepA (12.1 %); oqxA and oqxB (5.5 %). We conclude that there is a high frequency of Plasmid-mediated quinolone resistance genes in Escherichia coli isolates from clinical samples in South-Eastern Nigeria. These could be responsible for the high incidence of quinolone resistance reported in Enugu. There is a need for whole-genome sequencing to map out all resistance genes.
Keywords:
- Escherichia coli
- quinolone antibiotics
- plasmid
- antibiotics
- quinolone genes
How to Cite
Anayo, U. U., Okechukwu, N. E., Ngozi, O. F., Aisida, S. O., & Anyachukwu, C. C. (2023). Multiple Resistance in Clinical Isolates of Escherichia coli and Plasmid-mediated Quinolone Resistance Genes. Annual Research & Review in Biology, 38(2), 1-11. https://doi.org/10.9734/arrb/2023/v38i230568
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Moumoni A, et al. Quinolone Resistance (qnr) genes in fecal carriage of extended Spectrum beta-lactamases producing Enterobacteria isolated from children in Niger. Current Reasearch in Microbiology and Biotechnology. 2017;5:953–957.
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Firoozeh F, Zibaei M, Soleimani-Asl Y. Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran. Journal of Infection in Developing Countries. 2014;8:818–822. DOI: 10.3855/jidc.3746
Pallecchi L, Riccobono E, Mantella A, Bartalesi F, Sennati S, Gamboa H, et al. High prevalence of qnr genes in commensal enterobacteria from healthy children in Peru and Bolivia. Antimicrobial Agents and Chemotherapy. 2009;56:26 DOI: 10.1128/AAC.01722-08
Robicsek A, Strahilevitz J, Jacoby GA, Macielag M, Abbanat D, Chi HP et al. Fluoroquinolone-modifying enzyme: A new adaptation of a common aminoglycoside acetyltransferase. Nature Medicine. 2006;12:83–88.
Available:https://doi.org/10.1038/nm1347.
Ogbolu DO, Alli AO, Anorue MC, Daini OA, Oluwadun A. Distribution of plasmid-mediated quinolone resistance in Gram negative bacteria from a tertiary hospital in Nigeria. Indian Journal of Pathology and Microbiology. 2016;9:322–3. DOI: 10.4103/0377-4929.188108.
Zaman SB, Hussain MA, Nye R, Mehta V, Mamun KT, Hossain N. A Review on antibiotic resistance: Alarm bells are ringing. Cureus. 2017;9:e1403. DOI: 10.7759/cureus.1403
WHO, "Antimicrobial resistance. Global report on surveillance." World Health Organization; 2014. [Online].
Available:https://doi.org/10.1007/s13312-014-0374-3.
Penders J, Stobberingh EE, Savelkoul PHM, Wolffs PFG. The human microbiome as a reservoir of antimicrobial resistance. In Frontiers in Microbiology; 2013. Available:https://doi.org/10.3389/fmicb.2013.00087.
Etienne M, Lefebvre E, Frebourg N, Hamel H, Pestel-Caron M, Caron F, et al. Antibiotic treatment of acute uncomplicated cystitis based on rapid urine test and local epidemiology: Lessons from a primary care series. BMC Infect Dis. 2014;14:137. DOI: 10.1186/1471-2334-14-137
Martín-Gutiérrez G, Rodríguez-Martínez JM, Pascual Á, Rodríguez-Beltrán J, Blázquez J. Plasmidic qnr genes confer clinical resistance to ciprofloxacin under urinary tract physiological conditions. Antimicrobial Agents and Chemotherapy. 2017;61:e02615-16. Available:https://doi.org/10.1128/AAC.02615-16
Pham TDM, Ziora ZM, Blaskovich MAT. MedChemComm Quinolone antibiotics. Med. Chem. Commun. 2019;10:1719–1739. Available:https://doi.org/10.1039/C9MD00120D
Sárközy G. Quinolones: A class of antimicrobial agents. Veterinarni Medicina. 2001;46(9-10):257–274.
Tarchouna M, Ferjani A, Marzouk M, Guedda I Boukadida J. Prevalence of plasmid-mediated quinolone resistance determinants among clinical isolates of Escherichia coli in a Tunisian hospital. International Journal of Current Microbiology and Applied Sciences. 2015; 4:195–206. DOI: 10.4236/aim.2021.1110041
Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular characterization of multidrug-resistant Escherichia coli isolates from bovine clinical mastitis and pigs in the Vojvodina Province, Serbia. Microbial Drug Resistance. 2017;24:95–103. DOI: 10.1089/mdr.2017.0016
Rodríguez-Martínez JM, Cano ME, Velasco C, Martínez-Martínez L, Pascual Á. Plasmid-mediated quinolone resistance: An update. Journal of Infection and Chemotherapy. 2011;17:149–182. DOI: 10.1007/s10156-010-0120-2.
Aldred KJ, Kerns RJ, Osheroff N. Mechanism of quinolone action and resistance. Biochemistry. 2014;53:1565–1574. DOI: 10.1021/bi5000564
Howe RA, Andrews JM. BSAC standardized disc susceptibility testing method (version 11). Journal of Antimicrobial Chemotherapy. 2012;67: 2783–2784. DOI: 10.1093/jac/dks391
Salah FD, Soubeiga ST, Ouattara AK, Sadji AY, Metuor-Dabire A, Obiri-Yeboah D, Banla-Kere A, Karou S, Simpore J. Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in in Lomé, Togo. Antimicrobial Resistance and Infection Control. 2019; 8: 1-8. DOI: https://doi.org/10.1186/s13756-019-0552-0.
Ramírez-Castillo F Y, Moreno-Flores A C, Avelar-González F J, Márquez-Díaz F, Harel J, Guerrero-Barrera A L, An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: Cross-sectional study. Annals of Clinical Microbiology and Antimicrobials. 2018;17:34. DOI: 10.1186/s12941-018-0286-5.
Ciesielczuk H, Hornsey M, Choi V, Woodford N, Wareham DW. Development and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance determinants. Journal of Medical Microbiology. 2013:62;1823–1827. DOI: 10.1099/jmm.0.064428-0
Yang T, Zeng Z, Rao L, Chen X, He D, Lv L, Wang J, Zeng L, Feng M, Liu J H. The association between occurrence of plasmid-mediated quinolone resistance and ciprofloxacin resistance in Escherichia coli isolates of different origins. Veterinary Microbiology. 2014;170;89- 96. Available:https://doi.org/10.1016/j.vetmic.2014.01.019
Moumoni A, et al. Quinolone Resistance (qnr) genes in fecal carriage of extended Spectrum beta-lactamases producing Enterobacteria isolated from children in Niger. Current Reasearch in Microbiology and Biotechnology. 2017;5:953–957.
Hong BK, Chi HP, Chung JK, Kim EC, Jacoby GA, Hooper DC. Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. Antimicrobial Agents and Chemotherapy. 2009;53:639–645. DOI: 10.1128/AAC.01051-08
Firoozeh F, Zibaei M, Soleimani-Asl Y. Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran. Journal of Infection in Developing Countries. 2014;8:818–822. DOI: 10.3855/jidc.3746
Pallecchi L, Riccobono E, Mantella A, Bartalesi F, Sennati S, Gamboa H, et al. High prevalence of qnr genes in commensal enterobacteria from healthy children in Peru and Bolivia. Antimicrobial Agents and Chemotherapy. 2009;56:26 DOI: 10.1128/AAC.01722-08
Robicsek A, Strahilevitz J, Jacoby GA, Macielag M, Abbanat D, Chi HP et al. Fluoroquinolone-modifying enzyme: A new adaptation of a common aminoglycoside acetyltransferase. Nature Medicine. 2006;12:83–88.
Available:https://doi.org/10.1038/nm1347.
Ogbolu DO, Alli AO, Anorue MC, Daini OA, Oluwadun A. Distribution of plasmid-mediated quinolone resistance in Gram negative bacteria from a tertiary hospital in Nigeria. Indian Journal of Pathology and Microbiology. 2016;9:322–3. DOI: 10.4103/0377-4929.188108.
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