Ethylacetate Flavonoid Bio-compounds of Honey with Mitigating Anti-hyperlipidemic and Antioxidant Properties in Carbohydrate and Lipid Enriched Diets – Obese Rats
Annual Research & Review in Biology,
Page 1-23
DOI:
10.9734/arrb/2023/v38i930603
Abstract
Honey is a rich material source of medicinal nutrients. This study investigated the hypolipidemic and antioxidant effects of honey, 50% fresh lime juice, and 50% honey (MIX) and ethylacetate flavonoid-rich fraction of honey (EAFH) in carbohydrate and lipid-enriched diets-obese rats.
At phase 1, 54 male neonate Wistar albino rats were, divided into 3 groups of 18 rats. Groups 2 and 3 were fed a carbohydrate-enriched diet (CHD) and lipid-enriched diet (LP) for 14 days, and rats with Lee index ≥ 0.3 were considered obese. Rats (phase-2) were regrouped into 7 groups of 6 rats, and treated with honey, MIX, and EAFH. Flavonoids bio-compounds in EAFH characterized by HPLC (High-performance liquid chromatography) include; gallic acid, epigallocatechin, napthoresorcinol, and quercetin. Lee's index after obesity induction was ≥ 0.3. Adiposity index, diet intake, and body and organ weight of obese rats were significantly (p < 0.05) reduced after honey, MIX, and EAFH treatments compared to control. Significant (p < 0.05) decreased concentrations of glucose, leptin, insulin, low-density lipoprotein (LDL), total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), 3-Hydroxy-3-methylglutaryl (HMG) coenzyme A (CoA) reductase activity (HMGCOARA), atherogenic risk index (ARI) and coronary risk index (CRI) and increase in high-density lipoprotein (HDL) after treatment with honey, MIX and EAFH was observed compared control and AOI. Antioxidant parameters of obese rats were significantly (p < 0.05) improved compared to control and AOI rats. Honey could serve as a model pharmacotherapy for treating dyslipidemia and oxidative stress linked to obesity.
Keywords:
- Hypolipidemic
- hyperlipidemic
- flavonoids
- obesity
- hypoglycemic
- hyperglycemia
How to Cite
Alexander, I., Elijah, P. J., & Uzoma, N. O. (2023). Ethylacetate Flavonoid Bio-compounds of Honey with Mitigating Anti-hyperlipidemic and Antioxidant Properties in Carbohydrate and Lipid Enriched Diets – Obese Rats. Annual Research & Review in Biology, 38(9), 1-23. https://doi.org/10.9734/arrb/2023/v38i930603
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Mariarosaria B, Stefania D. Anti-Obesity Effects of Polyphenol Intake: Current Status and Future Possibilities. Inter. J. Mol. Sc. 2020; 20(5642):1-24.
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Idoko A, Ikpe VPO, Nelson NO. Effects of Lime Juice and Honey on Lipid Profile of Cholesterol Enriched Diet Fed Rat Model. Annual Research and Review in Biology. 2017;20(3):1-10.
Suhana S, Francis KE, Fuzina NH. Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gain and Improves Obesity-Related Parameters in High Fat Diet Induced Obese Rats. Evi. Comp. Altern. Med. 2017:1-9.
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Item JA, Chidimma EI, Godwin EE. Effect of long-term feeding of the Obudu natural honey and table sugar-sweetened diets on obesity and pro-inflammatory biomarkers in rats. BMC Nutrition. 2020;6(3):1-11.
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Fuad A, Fadi A, Wael AA, Hammad KA, Saleh A, Ghassab MA, Haya JAS. HPLC Analysis of Chemical Composition of Selected Jordanian Medicinal Plants and their Bioactive Properties. Orien J Chem. 2018;34(5):2397-2403.
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Nakagawa T, Ukai K, Ohyama T. Effects of chronic administration of sibutramine on body weight, food intake and motor activity in neonatally monosodium glutamate-treated obese female rats: relationship of antiobesity effect with monoamines. Expe. Animals. 2000;49:239–249.
Rotimi OA, Olayiwola IO, Ademuyiwa O. Effects of fibre-enriched diets on tissue lipid profiles of MSG obese rats. Food Chem. Toxicology. 2012;50:4062–4067.
Kamal MSA, Ghazali AR, Yahya NA. Acute toxicity study of standardized Mitragyna speciosa korth aqueous extract in Sprague dawley rats. J. Plant Study. 2012;1(2): 404–409.
Daniel EU, Item JA, Eyong UE. African walnuts attenuate ectopic fat accumulation and associated peroxidation and oxidative stress in monosodium glutamate-obese Wistar rats. Biomed. Pharmaco. 2020; 124(109879):1-10.
Idoko A. Exploitative Beneficial Effects of Citrus Fruits. In: Citrus - Health Benefits and Production Technology. Muhammad Sajid, Amanullah (Eds). IntechOpen. 2019; 32-55.
Shen W, Xu Y, Lu YH. Inhibitory effects of Citrus flavonoids on starch digestion and antihyperglycemic effects in HepG2 cells. J. Agric. Food Chem. 2012;60(38):9609-9619.
Burke AC, Sutherland BG, Telford DE. “Intervention with citrus flavonoids reverses obesity and improves metabolic syndrome and atherosclerosis in obese Ldlr-/- mice, J. Lip. Res. 2018; 59(9):1714–1728.
Waleed FA. Nutritional benefits of citrus fruits. Am. J. Biomed. Sc. Res. 2019;3(4): 1-4.
Pan MH, Wu JC, Ho CT. Antiobesity molecular mechanisms of action: Resveratrol and pterostilbene. BioFact. 2018;44:50–60.
Tan Y, Chang SKC. Digestive enzyme inhibition activity of the phenolic substances in selected fruits, vegetables and tea as compared to black legumes. J. Funct. Foods. 2017;38:644–655.
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