Open Access Case study

Desmoplastic Ameloblastoma in Maxilla: A Report of Rare Case with Review of Literature and Classic Histopathology

Vidya G. Doddawad, S. Shivananda, Pradeep Subbaiah, Divya Rao

Annual Research & Review in Biology, Page 1-7
DOI: 10.9734/ARRB/2017/38186

Ameloblastoma is a very rare odontogenic tumor of the oral cavity, with different histological variants. One of the types of ameloblastoma is desmoplastic ameloblastoma (DA) which has 4-5% of incidence. Here we review and reported the desmoplastic ameloblastoma is the least of occurrence of all the variants of ameloblastoma. The uniqueness of this lesion can be further enlightened with respect to its site of occurrence, the radiographic feature and the histological appearance different from the classical type of ameloblastoma. This case report focuses on a DA that occurred in the maxilla of 25-year-old women and explains about clinical, CT scan, histopathological finding, and treatment plan. The patient is undergoing routine follow-up and is presently free of disease.

From this case, the clinicians should remember to consider the desmoplastic ameloblastoma as differential diagnosis, if a patient complaint of swelling in and around the premolar region of the maxilla.

Open Access Original Research Article

The Fish Population Size of Tepetitlan Reservoir (Mexico) is at a Critical State

Daniel Aguilar-Ramírez, Patricia Devezé-Murillo, Juan Acosta-Jimeno, José Alfredo Villagómez-Cortés

Annual Research & Review in Biology, Page 1-9
DOI: 10.9734/ARRB/2017/37307

Aims: To determine the fish population structure of the Tepetitlan reservoir in the state of Mexico, Mexico, identifying the present species, distribution, age, length and weight.

Study Design: Convenience sampling.

Place and Duration of Study: Tepetitlan reservoir in the state of Mexico, Mexico, from April to September 2011.

Methodology: Each month, at least 200 fish were used for morphological measurements (weight, total length, standard length, max height and perimeter) and reproductive traits such as sex and gonadal maturity stage. Descriptive statistics were used to characterize the population structure, age and size of first capture. Growth parameters, natural mortality, instantaneous fishing mortality rate, exploitation rate, yield per recruit potential growth model were all calculated by empirical equations and formulas. Differences between height and weight by sex were explored by one-way analysis of variance defining weight as a covariate.

Results: The Tepetitlan reservoir has a small population of shortfin silverside (Chirostoma humboldtianum) with average catch sizes of 11 cm, and rare presence of axolotls (Ambystoma mexicanum) and marbled crayfish (Procambarus sp). The fishing effort of 0.53 and exploitation rate of 0.44 indicate that the reservoir is used within its tolerance limits. The compressed size of the populations, potential problems of inbreeding and reduced physical space for the species pushes the habitat to a fragile situation for their permanence.

Conclusion: Tepetitlan reservoir has a dramatically small population of shortfin silverside (Chirostoma humboldtianum), and an extremely small population of axolotls (Ambystoma mexicanum) and marbled crayfish (Procambarus sp).

Open Access Original Research Article

Investigation of Novel Tetrahalometallate Complexes of Cetrimonium Bromide Surfactant against in vitro Human Tumour Cell Lines of Lung, Colon and Liver

Hanan El-Sharkawy Ali Ahmed, Mohamad Fahmy Zuky, Abdulfattah Mohsen Badawi

Annual Research & Review in Biology, Page 1-9
DOI: 10.9734/ARRB/2017/38437

The synthesis of the tetrahalo Cu(II) and tetrahalo Zn(II) metal complexes with two cetrimonium bromide (CTAB) ligands is reported. Potentiometric studies showed that these complexes in aqueous solution showed no metal release, thus accounting for their high in vitro toxicity against three human cancer cell lines: A-549 (non-small cell lung carcinoma), HCT-116 (colon carcinoma cells), and HepG-2 (Hepatocellular carcinoma cells). The tetrahalo Cu(II) and Zn(II) metal complexes were synthesized by solid state grinding. Metal complexes of chelating CTAB with metal ion were studied on the basis of FT-IR, 1H-NMR and atomic absorption spectroscopic data. The tetrahalo Cu(II) and Zn(II) metal complexes induced cancer cell apoptosis. The tetrahalo complex of Cu(II) or Zn(II) inhibited in vitro the growth of three tumor cell lines at low concentrations. The tetrahalo copper(II) complex displayed against A-549 (non-small cell lung carcinoma) human cancer cell lines, IC50 values in mM range was similar to that of the antitumor drug cis-platin and they are considered for further stages of in vitro screening as potential antitumor activity.

Study Design: Antiproliferative activities were evaluated using a SRB and MTT assays. Effects on the cell cycle were assessed by flow cytometry and on apoptosis-related proteins (active caspase-3, -8 and -9, procaspase-3, -8, -9, PARP, Bid, Bcl-xL and Bcl-2) by Western blotting. Cu(II) and Zn (II) metal complexes displayed high inhibition potency against A-549 (non-small cell lung carcinoma), HCT-116 (colon carcinoma cells), and HepG-2 (Hepatocellular carcinoma cells).

Place and Duration of Study: Regional Center Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt, between March 2016 and June 2016.

Open Access Original Research Article

Effect of Chronic Oral Administration of Ruta montana L. Areal Part Extract on Fertility Potential in Albino Rats

Mounira Merghem, Saliha Dahamna, Kaoula Samira, Seddik Khennouf

Annual Research & Review in Biology, Page 1-8
DOI: 10.9734/ARRB/2017/38608

Aims: The objective of the present study is to evaluate the effect of the aqueous extract of Ruta montana L. on the fertility in adult male and female rats.

Methodology: Forty healthy adult rats of both sexes were divided into 4 Groups of 10 rats each. During the treatment period, 100, 300 and 600 mg/kg/day of the extract were orally administered to Groups I, II and III respectively, while the control group received distilled water and served as control. The daily administration was carried out for a period of 90 days.

Results: The results did not show any significant change in ovaries weight. However, a significant decrease in testis, epididymis, and seminal vesicles weights was detected, as well as a reduction in the number and the motility of spermatozoids in rats treated with the doses of 300 and 600 mg/kg.

Conclusion: A dose of 300 mg/kg and 600 mg/kg of aqueous extract of Ruta Montana L. was able to cause a decrease in sexual organs weight of male rats and in the number and the motility of spermatozoids.

Open Access Original Research Article

Weather Based Pest Forewarning Model for Major Insect Pests of Rice – An Effective Way for Insect Pest Prediction

Manikandan Narayanasamy, J. S. Kennedy, V. Geethalakshmi

Annual Research & Review in Biology, Page 1-13
DOI: 10.9734/ARRB/2017/37365

Weather parameters viz., Temperature, rainfall, relative humidity, sunshine hours and wind speed are the major weather elements determining the insect pests’ occurrence. Weather based forewarning models are widely utilized in the integrated pest management system as a tool which do not cause any harm to the predators and also cuts down environmental pollution. Considering this, an attempt was made to predict the population occurrence of Yellow Stem Borer (YSB), Brown Planthopper (BPH) and Rice Leaffolder (RLF). Generalized Linear Model (GLiM) was developed for YSB, BPH and RLF for predicting the population at a given time. The results of chi square test revealed that, there are many other factors which affect the amount of light trap catches of the insects apart from weather parameter. The predictability of the equation can be increased if the weather factors are combined with the other factors (variety, soil, fertilizer application, etc.,) in developing the model.