Open Access Original Research Article

Kinetic and Isotherm of Mn(II) Biosorption by Lyophilized Cells of Proteus penneri 2120 (KY712431) Isolated from Industrial Wastewater

Abdel-Hamied M. Rasmey, Alshimaa K. Youssef

Annual Research & Review in Biology, Page 1-14
DOI: 10.9734/ARRB/2018/38993

This study aimed to isolate and identify a novel bacterial isolate potential resistant to Mn2+ as well as to investigate the biosorption isotherms of Mn2+ removal from aqueous solutions by the freeze-dried biomass of this bacterium. Sixty three manganese resistant bacterial isolates were recovered from 20 industrial wastewater samples. Interestingly, among them, the isolate number 2120 was able to resist up to 140 ppm of Mn2+ and was selected for the further processes. This isolate was phenotypically characterized and identified by 16S rRNA gene sequencing as Proteus penneri and assigned accession number KY712431 in the GenBank database. The effects of pH and contact time on the biosorption process were studied and optimum pH for biosorption equilibrium was 6 while the optimum contact time was 30 min at room temperature. The maximum adsorption capacity (qmax) of Mn2+ removal from aqueous solutions by the freeze-dried biomass of Proteus penneri 2120 was 175.4 mg/g. According to Freundlich and Langmuir models, the correlation coefficients (R2) were 0.9977 and 0.5525, respectively. Therefore the studied biosorption isotherms are fit well with Freundlich model rather than the Langmuir model. Our findings suggest that the dried biomass of the isolate Proteus penneri 2120 is potentially applicable for manganese metal ion removal from the industrial waste water.

Open Access Original Research Article

Comparative Studies of the Impacts of Freshwater, Cultivated and Preserved Tiger Shrimps on Consumers’ Health

Isibor Patrick Omoregie, Izegaegbe Joshua Idowu, Igbinovia Joan Osahenrunmwen, Obafemi Dorcas Yemisi, Oluowo Elohor Freeman

Annual Research & Review in Biology, Page 1-13
DOI: 10.9734/ARRB/2018/38069

Aim: The study was aimed at comparing the levels of heavy metals in Tiger shrimps- Penaeus monodon from different source categories, to ascertain the impacts of the selected heavy metals on the consumers’ health.

Hypothesis: Heavy metals in blood serum of shrimp consumers were accumulated solely from the shrimps.

Methodology: Tiger shrimps obtained from Ekpan Creek, Shrimp industry and shrimp farm were tested for metals (iron, manganese, nickel, and lead) concentrations using a Perkin Elmer 3110 model Atomic Absorption Spectrophotometer (AAS). Blood samples of respondents were analyzed for the levels of iron, manganese, nickel and lead using Inductively Coupled Plasma MassSpectrometer (ICP-MS). Analysis of Variance (ANOVA) was used to test for the significant differences in the heavy metals in the shrimps and consumers’ blood samples at probability level of 0.05. Duncan Multiple Range (DMR) test was used to identify the source of variance using SPSS version 19.1. Health Risk Indices of heavy metals were noted as significant from 1 and above and insignificant at below 1.

Results: The shrimps exhibited different trends of heavy metals according to their sources. Results showed that the safety in consumption of the shrimps was is in the order of cultivate (pond) shrimps > freshwater shrimps > preserved shrimps. Shrimp consumption had corresponding impacts on the health of the consumers. Processed shrimps should be properly examined for fitness of consumption prior to marketing, while chemicals such as sodium bisulfate, tripolyphosphate should be discouraged.

Conclusion: Metallic construction materials should be discouraged and stringent water quality monitoring is recommended in Tiger shrimp aquaculture while incorporating adoption of environment friendly agricultural practices. Perturbed aquatic environments such as Ekpan Creek require protection from anthropogenic activities and impactful remediation process with a view to protecting the Tiger shrimps and other aquatic biota.

Open Access Original Research Article

Factors Affecting Microbial Oil Accumulation by Oleaginous Yeast

Dalia M. Mohammed, Fatma H. Abd El Zaher, Enass A. Hassan, H. K. Abd El Maksoud, E. M. Ramadan

Annual Research & Review in Biology, Page 1-12
DOI: 10.9734/ARRB/2018/38425

The growth performance and intracellular lipid production potentiality of two identified yeast stains Candida tropicalis (S5) and Issatchenkia orientalis (D5) in N-limited medium under different carbon, nitrogen sources, carbon/nitrogen ratio and temperature was studied. Among all carbon sources, glucose was the best in lipid weight, lipid content, lipid yield and lipid productivity. The increases in cell biomass reached its maximum value by use of yeast extract in Candida (8.62 gL-1) and 10.25 gL-1 for Issatchenkia using peptone. However, urea was the preferable N source for lipid production in both strains. On the other hand, KNO3 proved to be the lowest values treatment in the cell biomass and lipid production for both strains. The interaction treatments yeast extract + (NH4) SO4 was convenient for oil yield parameters in Candida. The data showed that the maximum cell biomass was obtained at 54 and 100 C/N ratio for Candida and Issatchenkia, respectively. The data also exhibited that designated sharp increases in lipid weight, lipid content and lipid productivity were developed by the two yeast strains reached their maximum values at C/N ratio 115 (1.0 gL-1, 27.77%, 0.20 gL-1) for Candida tropicalis and C/N ratio 100 (3.37 gL-1, 30.92%, 0.67 gL-1) for Issatchenkia orientalis. Further increase in C/N ratio resulted in drop in lipid weight; this drop was slight in Candida above 115, whereas it was drastically in Issatchenkia after C/N 100. The result of effect of temperature degree on cell biomass and lipid production showed that the temperature degree of 30°C was the best for yeast growth and also for lipid production.

Open Access Review Article

Survey on Identification Tools for Hepatocellular Carcinoma- A Review

G. Rajesh, A. Selwin Mich Priyadharson

Annual Research & Review in Biology, Page 1-25
DOI: 10.9734/ARRB/2018/37827

Aims: The main objective of this paper is to review the technologies used for the detection of Hepatocellular Carcinoma.

Study Design: Convective cooling protects the cancer cells from thermal destruction and decreases the necrosed volume. A major objective of the method development is to achieve a virtually complete necrosis of tumors close to major blood vessels and to avoid blood vessel damage and, hence, the  needed treatment planning.

Place and Duration of Study: We found from this three-dimensional three-field coupling study that in large blood vessels, both convective cooling and acoustic streaming may change the temperature considerably near the blood vessel. Acoustic streaming velocity magnitude can be several times larger than the blood vessel velocity.

Methodology: Different methods and techniques were proposed so far in the automatic detection of Hepatocellular Carcinoma. However the performance of the technologies till now not completely matched to the performance of human expert.

Results: This review paper have analyzed the recent technologies in Liver Cancer Identification (24%), Liver Tumor Risks (16%), MR Imaging (22%), Liver Tumor Prevention (16%) and Liver Tumor Therapy (18%).  The results presented in the current work can be further used to construct a surgical planning platform.

Conclusions: Also we give some directions about the technologies and this can be useful for the researches to develop a new technology for the detection of Hepatocellular Carcinoma.

Open Access Review Article

Ofada Rice Identity, Physical Qualities and Processing Technology Options for Upgrading: A Review

O. O. Adekoyeni, S. A. Fagbemi, A. R. Ismaila

Annual Research & Review in Biology, Page 1-9
DOI: 10.9734/ARRB/2018/38938

The main aim is to provide an overview on identity of Ofada rice and technology options for quality upgrading. Ofada rice is one of the popular indigenous Nigerian rice with complicated identity. This local rice is robust with red/brown stripes and characterised with unappealing appearance causing downgrading or rejection. Physical qualities play a significant role in the determination of consumer preference. Aside from genetic, non genetic factors contribute substantially to the physical quality of rice. The quality differences between Nigerian rice and imported rice varieties have been of concern to the domestic rice industry. Careful examination of research findings have lent strength to the influence of post harvest rough rice handling and processing on the quality of Ofada rice. Most of these previous researches to improve Ofada rice quality focused on a particular processing unit operation (soaking condition and time, parboiling temperature, drying temperature and storage duration). Identity of Ofada rice and its physical attributes are re-examined. The traditional primary and secondary processing operations involved are also revisited. Appropriate technological options for improving physical qualities for better product quality and acceptability were compounded.