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Open Access Original Research Article

Growth and Yield Production of Canola as Affected by Organic and Mineral Fertilizers Application under Drought Stress Conditions

Saied El Sayed, Farid Hellal, Hanan H. Abdel-Kader

Annual Research & Review in Biology, Page 1-13
DOI: 10.9734/arrb/2021/v36i130328

Field experiment were carried out at the Research and Production Station of the National Research Centre, Nubaria region, Behiera Governorate, Egypt during 2019/2020 to study the effects of compost and zinc foliar application on growth and yield production of canola under drought condition. The water stress treatments (75 and 40% water holding capacity, WHC) occupied the main plots and compost treatments at the rates (0, 2, 4 and 6 ton fed-1) were allocated at random in the sub-plots with the foliar application of zinc at a rate of 0, 50 and 100 ppm. Data noticed that increase compost rate associated with increasing the SPAD red and RWC under both irrigation treatments where 75%WHC scored the highest values than 40 %WHC. Results indicated that increasing compost rates was associated with increasing of canola characters plant height, number of branch, number of pods and 1000 seeds weight as well as, seed yield, biological yield as affected by compost and zinc application rates and their interaction under normal and stress treatments. Also, data illustrated that stress tolerance indices (SSI, STI, GMP, YI, YSI, MP, TOL and HM) of canola as affected by water stress and Compost application and Zinc sulphate. Data indicated that the highest values were attained after application compost at a rate of 6.0 ton fed-1 with a foliar spray ZnSO4 at a rate of 100 ppm. Results concluded that, application of compost and zinc is important for enhancing the growth parameter and seed production of canola under water stress condition.

Open Access Original Research Article

Chemical Constituents and Larvicidal Properties of n-Hexane Extract of Parinari excelsa Seeds

A. Dokubo, F. G. Obomanu, N. Ebere, G. I. Ndukwe

Annual Research & Review in Biology, Page 14-23
DOI: 10.9734/arrb/2021/v36i130329

The study was conducted to investigate the chemical compositions and larvicidal effect of n-hexane extract of Parinari excelsa seeds against fourth instar larvae of Culex mosquito after 24 h and 48 h exposure. The chemical composition of n-hexane extract of P. excelsa seeds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Larvicidal activity was performed following standard procedures of World Health Organization (WHO). GC-MS analysis showed that the seed extract of P. excelsa contained hexadecyl phenyl carbonate with highest percentage (7.502%, RT=26.39), followed by tetradecyl phenyl carbonate (5.77%, RT=25.90), 1-methyl cyclohex-3-enyldodecyl fumarate (5.70%, RT=24.58), decyl phenyl carbonate (4.70%, RT=28.64) and the lowest, octadecyl-2,2,2-trichloroethyl carbonate (0.62%, RT=13.71). The result showed significant (p<0.05) mortality of larvae  in 24 h and 48 h of exposure. However, the highest larval mortality  was recorded at 48 h exposure. Result of regression analysis indicated that mortality rate positively correlated with concentration having a regression coefficient (R) close to one in each exposure case. The estimated lethal concentrations (LC50) for 24 h and 48 h exposure were 2.056±0.176 µg/ml and 0.429±0.150 µg/ml respectively. This indicates that larvicidal activity recorded for 48 h exposure was 4.8 times more than that recorded for 24 h exposure. The study demonstrated that n-hexane extract of P. excelsa seeds exhibited larvicidal potential and can be utilized as biopesticides to minimize the multiplication of mosquitoes that transmit vector borne diseases.

Open Access Original Research Article

Development of Induced Breeding Technique for Freshwater Fish Gobi, Glossogobius giuris (Hamilton, 1822) Using Pituitary Gland (PG) Extract

Selina Yeasmine, M. Aminur Rahman, Md. Sarower-E-Mahfuj, Sonia Sku, Md. Nazmul Hossen, M. Anisur Rahman, Md. Fazlul Awal Mollah

Annual Research & Review in Biology, Page 24-35
DOI: 10.9734/arrb/2021/v36i130330

This study aimed to establish induced breeding technique of freshwater fish gobi (Glossogobius giuris), using the pituitary gland (PG) extract. Six experiments were conducted at the Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh. First breeding trial was conducted in June using 40, 45, and 50 mg PG kg-1 body weight of the female fish. None of the fish was ovulated in the 1st trial. Two more breeding trials were conducted in July and August, using 6 mg (T1), 8 mg (T2) and 10 mg (T3) PG kg-1 body weight of female, and 3 mg (T4), 4 mg (T5) and 5 mg (T6) PG kg-1 body weight of male fish. After treatment, the ovulation rates were recorded as 56.33±1.53, 82.67±2.52 and 75.33±1.53% in July and 58.00±2.65, 94.67±1.53 and 78.33±1.53% in August under the treatments T1, T2 and T3, respectively. Hatching rates of eggs were observed as 52.00±4.36, 81.67±3.21 and 72.33±6.03% in July, and 54.67±3.23, 91.67±3.06 and 73.67±5.13% in August under the T1, T2 and T3, respectively. Hatching time was ranged from 35 to 48 h and after the absorption of yolk sac (60-72 h), they were survived well when fed with tubificid worms and mixed zooplankton. The female treated with the dose of 8 mg PG kg-1 body weight in August showed the best performance so far as the ovulation, fertilization and hatching rates were concerned, while the single dose of 4 mg kg-1 body weight of PG was found to be effective for male fish in both months. The findings obtained from the present study reveals that induced breeding of G. giuris, using PG extract is successful for large scale production of quality seed in captive condition, which would further facilitate towards the aquaculture production and biodiversity conservation of this important fish species to a greater extent.