Open Access Short communication

Pollen Morphology of Six Indonesian Begonia (Begoniaceae) Species

Hartutiningsih M. Siregar, . Sudarmono, Joko Ridho Witono, Hary Wawangningrum

Annual Research & Review in Biology, Page 7-13
DOI: 10.9734/arrb/2021/v36i530370

Aims: This study aims to determine pollen morphological differences in six Indonesian Begonia species.

Study Design: All fresh pollen grain of six Indonesian Begonia species were collected using a standard method and observed at SEM (Scanning Electron Microscope) Laboratory.

Place and Duration of Study: The pollen is collected from Bogor Botanic Gardens, then observed using an SEM in The Museum Zoologicum Laboratory, Research Center for Biology, Indonesian Institute of Sciences (LIPI), on August 2018.

Methodology: The results of SEM pollen ultrastructure observations were analyzed descriptively by describing images of size, P/E index, aperture, endoaperture, and ornamentation of pollen. The size of pollen morphology is calculated based on the length of the polar axis and the equatorial axis on a micrometer scale. Determination of the shape of pollen can be determined by comparing the length of the polar axis with the equatorial axis.

Results: All species examined share common pollen grain characters, i.e. isopolar and three zonocolporate. The longest polar axis (P) and equatorial diameter (E) presented by B. puspitae, whereas the shortest of P is shown by B. kudoensis and the shortest of E by B. sudjanae. Only B. hooveriana has prolate – perprolate aperture, whereas other species perprolate. Endoaperture types of B. puspitae, B. sudjanae, and B. hooveriana is lalongate, while the other species is lolongate. The coarsely striate ornamentation forms are presented by B. holosericeoides and B. natunaensis, whereas other species has fine striate ornamentation.

Conclusion: The size, P/E index, aperture, endoaperture, and ornamentation of pollen are not useful for section classification of six Indonesian Begonia species. Pollen morphology should be incorporated to other characters, such as morphological, cytological, and molecular characters for making delimitation of Begonia species.

Open Access Original Research Article

Determination of Free DNA (cfDNA) by RT-qPCR in Individuals in Sperm Alterations

Modou Mamoune Mbaye, Hasnae Zekhnini, Bouchra El Khalfi, Noureddine Louanjli, Mustafa Zakaria, Fatiha Elmellouli, Abdelaziz Soukri

Annual Research & Review in Biology, Page 1-6
DOI: 10.9734/arrb/2021/v36i530369

Previous studies have suggested that the presence of circulating nucleic acids (cell-free DNA) in seminal plasma may indicate disease states. However, the potential association between cell-free DNA (cfDNA) levels in seminal plasma and sperm fertility parameters has not yet been definitively determined.

In this study, we will compare seminal free DNA levels between normozoospermic samples and those from different pathologies related to characteristic parameters of sperm quality (asthenozoospermia, azoospermia, teratozoospermia, oligozoospermia and a few samples with a high fragmentation index) in order to detect a potential association between free DNA levels in seminal plasma and these different pathologies of male fertility.

The recovery of free DNA from our different samples was done with the MACHEREY-NAGEL NucleoSpin® kit. This kit allows isolation of DNA from cell-free biological fluids using rapid silica column procedures. The quantification of free DNA in our samples was performed by quantitative PCR (RT-qPCR).

Our results showed a significant difference in the level of free seminal DNA between normozoospermic samples and oligozoosperimic, teratozoosperimic, azoosperimic samples and those with a high DNA fragmentation index. On the other hand, no significant difference in the level of seminal free DNA was noted between normozoospermic and asthenozoospermic samples.

These results suggest that seminal free DNA may be an important biomarker in the assessment of human sperm fertility.