Determination of Minimum Inhibitory Concentration (MIC) of a PolyHexamethylene Biguanide (PHMB) Solution: A Potential Root Canal Irrigant
Chandki Rita *
Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.
Vineeta Nikhil
Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.
Sai Kalyan Surapaneni
Department of Conservative Dentistry and Endodontics, Rural Dental College, PIMS, Loni, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
Background: A polymeric biguanide ‘PolyHexaMethylene Biguanide’ (PHMB) was evaluated for its Minimal Inhibitory Concentration (MIC) against ATCC reference laboratory strain of Enterococcus faecalis (E. faecalis).
Methods: The MIC was determined using criteria’s laid down in DIN 58940-7 and 58940-8 and the corresponding supplementary sheets. Serial dilutions of PHMB were tested against E. faecalis in 96-well microtitre plates and MIC was read as the minimal concentration that allowed no visible growth.
Results: The MIC for PHMB against tested organism was found to be 2 gm/ L (0.2%).
Conclusion: Lower MIC value (2 gm/ L (0.2%)) of PHMB solution against E. faecalis is an indicator of its higher potency against the said microorganism and paves way to further research in its potential use as a root canal irrigant.
Keywords: E. faecalis, Minimum Inhibitory Concentration (MIC), Polyhexamethylene Biguanide (PHMB), Polyhexanide, Root canal irrigant.