Evaluation of Antioxidant and Free Radical Scavenging Activity of Tagetes patula
Deepshikha Kushwaha *
University Grant Commission, Department of Biochemistry and Biochemical Engineering, Sam Higgin Bottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad-211007, India.
Yashodhara Verma
University Grant Commission, Department of Biochemistry and Biochemical Engineering, Sam Higgin Bottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad-211007, India.
*Author to whom correspondence should be addressed.
Abstract
In present study, the preliminary screening of Tagetes patula contains many phytochemicals. These phytochemicals are able to reduce the oxidative stress in living organisms under adverse conditions. T. patula contains high ratio category of polyphenolic compounds such as phenol and flavonoids. Antioxidant activity of extracts was expressed as percentage of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals inhibition and IC50 values (μg/mL) ranged from 11.86 to 16.06%. The total phenolic content ranged from 30.26 to 80.08 mg/g of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentration varied from 30.00 to 65.00 mg/g, expressed as quercetin equivalents. In this study, phenolic content was quite higher in leaves as compared to flavonoids in flowers. The objective of this study to determined the antioxidant activity of Tagetes patula.
Keywords: Antioxidants, DPPH, flavonoids, phenol, Tagetes patula.