Plasma Lipoprotein Profile in Newly Hatched Chicks Following in Ovo Ghrelin Administration
Published: 2011-09-13
Page: 117-122
Issue: 2011 - Volume 1 [Issue 4]
Alireza Lotfi *
Ilkhchi Branch, Islamic Azad University, Ilkhchi, Iran.
Habib Aghdam Shahryar
Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran.
Mohammad Narimani-Rad
Ilkhchi Branch, Islamic Azad University, Ilkhchi, Iran.
*Author to whom correspondence should be addressed.
Abstract
Objective: The aim of this study was to investigation on effects of in ovo administrated exogenous ghrelin on plasma lipid profile in hatched broiler chicks.
Methodology: 250 eggs were obtained from commercial broiler breeder (Ross 308 strain) farm. the eggs were divided into five experimental groups; T1 or eggs without any injection (control), T2 or eggs in ovo injected with 50ng ghrelin at embryonic day-5, T3 or eggs in ovo injected with 100ng ghrelin at embryonic day-5, T4 or eggs in ovo injected with 50ng ghrelin at embryonic day-10, and T5 or eggs in ovo injected with 100ng ghrelin at embryonic day-10. Similar in ovo experiments were done for all of injected groups on day-5 or -10. At end of incubation, blood samples from each group were collected following chick decapitation and analyzed for determination of plasma lipoprotein concentrations.
Results: Exogenous ghrelin administration at different embryonic days couldn’t have any considerable effect on low density lipoprotein (LDL-C), very low density lipoprotein (VLDL-C) or high density lipoprotein (HDL)/LDL-C. Plasma HDL-C concentration had increase follow in ovo injection of 100ng ghrelin at day-5 (P<0.01) and had slight increase at day-10 (group T3 and T5).
Conclusion: As conclusion, in ovo administration of 100ng ghrelin at embryonic day-5 could elevate plasma HDL-C concentrations of newly-hatched chicks without any significant effect on LDL-C, VLDL or HDL-LDL ratio.
Keywords: Ghrelin, in ovo administration, lipid, plasma lipoproteins