Renal Protective Effect of Ginkgo biloba and L-carnitine Extracts against Pentylenetetrazol Induced Toxicity, Oxidative Stress, Injury and Proliferation Alternation in Epileptic Rats
Ehab Tousson *
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
Akaber T. H. Keshta
Department of Chemistry, Faculty of Science, Zagazig University, Egypt.
Youssef Hussein
Department of Anatomy and Embryology, Faculty of Medicine, Mutah University, Jordan and Department of Anatomy Faculty of Medicine, Zagazig University, Egypt.
Reda M. Fekry
Department of Chemistry, Faculty of Science, Zagazig University, Egypt.
Wafaa K. Abo-Ghaneima
Department of Chemistry, Faculty of Science, Zagazig University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Objectives: Ginkgo biloba leaf extract (GBLE) and L-carnitine extract are a commercial product used as a nutraceutic herbal widely. They have antioxidant and reactive oxygen species (ROS) scavenger. This study hypothesized that GB and L-carnitine may have protective effects against pentylenetetrazol (PTZ) induced oxidative damage in kidney in male albino rats.
Materials and Methods: A total of 80 male albino rats were equally divided into eight groups (G1, control; G2, GBLE; G3, L-carnitine; G4, Pentylenetetrazole; G5&G6, pre and post treated PTZ with L-carnitine groups respectively; G7&G8, pre and post treated PTZ with GBLE respectively). Results: Serum urea, creatinine, uric acid and cystatin c were significantly increased in PTZ group when compared with control. Also; kidney homogenate MDA and DNA fragmentation were increased when compared with control while, significant decrease in CAT, SOD, GST and TAC in PTZ when compared with control. On other hand pre and post-treatment with L-carnitine and GBLE improved these parameters.
Conclusions: The results revealed that; the treatment with L-carnitine improved these parameters more than GBLE and the results of post treatment were better than pre-treatment.
Keywords: Pentylenetetrazole, Ginkgo biloba, L-carnitine, oxidative stress, DNA damage, kidney rats.