Ex-vivo Acetylcholinesterase and Butyrylcholinesterase Inhibitory Activities Assay of G. asiatica and G. tiliaefolia (Tiliaceae) Leaves
Md. Kamrul Islam Joy
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
Nazma Akhter
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
Rawnak Reza
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
Mst. Sayma Rahman Antara
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
Md. Shariful Islam *
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
Celia-Vargas de la Cruz
Facultad de Farmacia y Bioquímica, Centro Latinoamericano de Enseñanza e Investigación de Bacteriología Alimentaria (CLEIBA), Universidad Nacional Mayor de San Marcos, Lima, Perú.
Md. Rakibul Islam
Department of Pharmacy, Southeast University, Banani, Dhaka, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Aims: Our study was carried out to appraise acetylcholinestrase (AChE) and butyrylcholinestrase (BChE) inhibitory activities of Grewia asiatica and Grewia tiliaefolia leaves extracts.
Study Design: For the purpose of these experiments the extracts were subjected to an ex-vivo study.
Place and Duration of Study: The study was carried out between June 2018 to December 2018 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh.
Methodology: In this study, cholinesterase inhibitory activities of different fractions of crude ethanol extract of both plants were examined using swiss albino mice at 300 mg/kg b.w. dose. We determined anti-acetylcholinestrase (AChE) and anti-butyrylcholinestrase (BChE) activities using slightly modified Elman coupled enzyme assay.
Results: The highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by PEF and CLF of G. asiatica with the IC50 values were found to be 55.88 μg/ml and 26.14 μg/ml respectively whereas the highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by CLF of G. tiliaefolia.
Conclusion: The result of the present study on various fractions of these plants has a considerable anti-acetylcholinesterase and anti-butyrylcholinesterase activities which suggest its effectiveness against various neurodegenerative disorders.
Keywords: Free radicals, acetylcholinesterase, butyrylcholinesterase, G. asiatica, G. tiliaefolia.