Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties
A. O. Akintola *
Department of Science Laboratory Technology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
A. M. Azeez
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
B. D. Kehinde
Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
I. C. Oladipo
Department of Science Laboratory Technology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.
Keywords: Silver nanoparticles, Adansonia digitata, antimicrobial activity, nano particles, methanolic extract.