Gene Expression Endpoints Following Subchronic Thiamethoxam Exposure in Adult Male Rabbits
Ahmed R. Elsawasany
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 23, Academician Skryabin Street, Moscow, 109472, Russia and Faculty of Veterinary Medicine, Alexandria University, Arab, Republic of Egypt, Egypt.
Alexander A. Deltsov
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 23, Academician Skryabin Street, Moscow, 109472, Russia.
Osama S. El Okle
Faculty of Veterinary Medicine, Alexandria University, Arab, Republic of Egypt, Egypt.
Omnia I. El Euony
Faculty of Veterinary Medicine, Alexandria University, Arab, Republic of Egypt, Egypt.
Sergey V. Pozyabin
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 23, Academician Skryabin Street, Moscow, 109472, Russia.
Konstantin S. Ostrenko *
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 23, Academician Skryabin Street, Moscow, 109472, Russia and Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst, Dubrovitsy 60, Podolsk Municipal District, Moscow Region, 142132, Russia.
*Author to whom correspondence should be addressed.
Abstract
Aim: to investigate the sub chronic toxicity of thiamethoxam on some parameters of reproductive performance in adult male rabbits including gene expression of LDH-C4, FSHβ and LHβ and GnRHR.
Method: sixteen adult male Chinchilla rabbits were divided into two equal groups. Animals in the first group were treated orally with TMX at dose of 250 mg/kg body weight for 90 days. The second group was served as control.
Result: Obtained results showed that TMX increased the relative weight of some reproductive organs including testis and prostate. Hormonal analysis revealed that, TMX induced a significant elevation in the serum testosterone level, while the concentrations of FSH and LH hormones did not exhibit any alterations between treated and control groups. In addition, LDH-C4, FSHβ and LHβ and GnRHR genes were down regulated in TMX treated group.
Conclusion: Administration of thiamethoxam for 90 days in male rabbits induced a noticeable adverse effect on serum testosterone level and down regulated genes related to male rabbit reproductive performance.
Keywords: FSH, Gene expression, GnRHR, Hormones, LH, LDH-C4, Neonicotinoids, Testis, Thiamethoxam, sub chronic toxicity