Antioxidant, Anti-inflammatory and Cytotoxic Properties of Cancerol S, A Blend of Medicinal Plants, on Cancerous Cells in Culture
Marc Donald Wilfried Adico
Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, Burkina Faso and Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso.
Bagora Bayala *
Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, Burkina Faso, Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso, Ecole Normale Supérieure, Koudougou, Burkina Faso and Université Clermont Auvergne, Institute Génétique, Reproduction, Développement, UMR CNRS 6293, INSERM U1103, 28, Place Henri Dunant, BP38, F63001, Clermont-Ferrand, France.
Florencia Wendkuuni Djigma
Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, Burkina Faso and Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso.
Bapio Valerie Elvira Jean Telesphore Bazié
Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, Burkina Faso and Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso.
Paul Darga
Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso.
Jean-Marc Lobaccaro
Université Clermont Auvergne, Institute Génétique, Reproduction, Développement, UMR CNRS 6293, INSERM U1103, 28, Place Henri Dunant, BP38, F63001, Clermont-Ferrand, France.
Jacques Simporé
Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, Burkina Faso and Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aims: To evaluate in vitro the properties of a blend of medicinal plants called Cancerol S through its antioxidant, anti-inflammatory and cytotoxic potential on cancerous cells in culture.
Methodology: High-performance thin-film chromatography (HPTLC) and the assay test were used for phytochemical screening of Cancerol S. The antioxidant power of Cancerol s was evaluated by free radical inhibition tests (DPPH and ABTS) and the Iron III Reduction Power (FRAP). The inhibition tests of 15-LOX (15-lipoxygenase) and COX1/COX2 (cyclooxygenases) were used to evaluate the anti-inflammatory capacity of Cancerol S.
Results: The total polyphenols and flavonoids content of the extract were respectively 115.6 ± 0.1 mg EAG/g and 43.4 ± 0.1 mg EQ/g dry extract. The extract showed excellent antioxidant activity, with inhibition of DPPH (EC50 = 8.3 ± 0.7 μg/mL) and ABTS (EC50 = 36.0 ± 1.1 μg/mL), and also reduced ferric ion Fe3+ (2.67 ± 0.1 mmol EAA/g extract). In addition, the extract moderately inhibited 15-LOX, COX-1 and COX-2. The HeLa cell line of cervical cancer was more sensitive to Cancerol S (EC50 = 84.6 ± 5.2 μg/mL) than the prostate cancer cell line DU 145 (EC50 = 140.7 ± 7.8 μg/mL).
Conclusion: These properties give a rational basis for the use of Cancerol S in traditional medicine in Burkina Faso.
Keywords: Cytotoxic, antioxidant, anti-inflammatory, Cancerol S