Cytotoxic and Anti-inflammatory Properties of Two Medicinal Plant Recipes Decoctions, acphyx and chryfa, Used in Traditional Medicine of Burkina Faso
Boly Abdoul Gilchrist Laurent *
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Bayala Bagora *
Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso, Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso and Higher Normal School, Koudougou, Burkina Faso.; 01 BP 216 Ouagadougou 01, Burkina Faso.
Kaboré Sergine
Saint Thomas d'Aquin University (USTA), 06 BP: 10212 Ouagadougou 06, Burkina Faso.
Belemlilga Bonewendé Mohamed
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Ouédraogo Annick Madinatou
Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso and Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso.
Yaro Boubacar
Saint Thomas d'Aquin University (USTA), 06 BP: 10212 Ouagadougou 06, Burkina Faso.
Yabre Zacqueline
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso and Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso.
Koala Moumouni
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Ilboudo Dénise
Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso and Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso.
Sombié Charles
Drug Development Laboratory (LADME), Center for Training, Research and Expertise in Drug Sciences (CEA-CFOREM), Doctoral School of Health Sciences (ED2S), Joseph KI-ZERBO University, BP 7047, Burkina Faso.
Nadembega Pascal
Department of Traditional Medicine, Ministry of Healt, 01 BP 216 Ouagadougou 01, Burkina Faso.
Compaoré Jean-Marie
Department of Traditional Medicine, Ministry of Healt, 01 BP 216 Ouagadougou 01, Burkina Faso.
Darga Paul
Department of Traditional Medicine, Ministry of Healt, 01 BP 216 Ouagadougou 01, Burkina Faso.
Zohoncon Théodora
Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso, Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso and Saint Thomas d'Aquin University (USTA), 06 BP: 10212 Ouagadougou 06, Burkina Faso.
Traore Aristide
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Ouédraogo Noufou
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Ouédraogo Sylvin
Institute for Research in Health Sciences (IRSS/CNRST), 03 BP7192 Ouaga 03, Burkina Faso.
Semdé Rasmane
Drug Development Laboratory (LADME), Center for Training, Research and Expertise in Drug Sciences (CEA-CFOREM), Doctoral School of Health Sciences (ED2S), Joseph KI-ZERBO University, BP 7047, Burkina Faso.
Simporé Jacques
Labiogenne, Joseph KI-ZERBO University, BP 7047, Burkina Faso, Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso and Saint Thomas d'Aquin University (USTA), 06 BP: 10212 Ouagadougou 06, Burkina Faso.
Guissou Innocent Pierre
Pietro Anignoni Biomolecular Research Center (CERBA), 01 BP 216 Ouagadougou 01, Burkina Faso and Saint Thomas d'Aquin University (USTA), 06 BP: 10212 Ouagadougou 06, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Aims: Cancer is a major public health problem worldwide and in Burkina Faso. This study aimed to evaluate the cytotoxic and anti-inflammatory properties of ACPHYX and CHRYFA, two medicinal plant recipes used against inflammatory diseases in traditional medicine in Burkina Faso.
Study Design: The anti-inflammatory and anti-proliferative activity on prostate and cervical cancer cells of a recipe orients on its possible anti-cancer properties. Then would help to realize ethnopharmacological evidence study for its validation or not as a phytomedicine against prostate or cervical cancer.
Place and Duration of Study: The studies were carried out in the laboratories of the Pietro Anignoni Biomolecular Research Center (CERBA) and the Health Sciences Research Institute (IRSS/CNRST) from December 2021 to March 2023 in Burkina Faso.
Methodology: An aqueous decoction of ACPHYX recipe and CHRYFA recipe, were made. The phytochemical groups of interest and the phenolic compound content of the extracts were determined. The antioxidant test was performed by the DPPH and FRAP methods. The cytotoxic activity on cancer cells was evaluated by the MTT test. Anti-inflammatory activity was assessed by pro-inflammatory enzyme inhibition tests (15-lipoxygenase, cyclooxygenases), anti-edema and antihistamine tests.
Results: ACPHYX decoction showed the best antioxidant activity by DPPH method (IC50 of 12.80 ± 0.5μg/mL). The chelation capacity evaluated by FRAP method was also better with ACPHYX decoction (1.94 ± 0.296 mmol EAA/g). ACPHYX decoction also showed the best cytotoxic activity on prostate cancer DU145 cells (175.5 ± 3.79 μg/mL) and cervical cancer HeLa cells (302.5 ± 64.36 μg/mL). In vitro, the extracts inhibited 60% edema at 400 mg/kg dose and 79.32% scratching inhibition at 600 mg/kg dose.
Conclusion: The strongest anti-inflammatory activity in vitro and in vivo was observed with ACPHYX extract. ACPHYX recipe compared to CHRYFA showed better cytotoxic and anti-inflammatory properties. Its use could help to treat tumor and inflammatory conditions through further studies.
Keywords: Acphyx, Chryfa, cytotoxicity, anti-inflammatory, Burkina Faso