In vitro Microrhizome Formation in Kaempferia parviflora
A. R. Zuraida *
Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
K. Fatin Liyana Izzati
Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
O. Ayu Nazreena
Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia.
Nurnadhira Omar
Faculty of Science and Technology, Islamic Science University of Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
*Author to whom correspondence should be addressed.
Abstract
Plantlets produced in vitro from rhizomes of Kaempferia parviflora were used as the source of explants for microrhizome development. Sixty percent of the plantlets formed microrhizomes in liquid medium supplemented with1 mg/L BAP+1 mg/L NAA, with 60 g/L sucrose. The treatment gave the highest fresh weight of microrhizomes, at 265 mg/plantlet. This optimized protocol is suitable for the commercial production of disease-free Kaempferia microrhizomes that can be stored and transported easily.
Keywords: Kaempferia parviflora, microrhizomes, medicinal plants, in vitro