Evaluation of Seedling Traits in M1 Generation of Sesame (Sesamum indicum L.) under Laboratory Conditions
M. R. Vaghasiya *
Department of Genetics and Plant Breeding, Junagadh Agricultural University, India.
G. K. Sapara
Main Oilseeds Research Station, Junagadh Agricultural University, India.
H. D. Gadhiya
Department of Genetics and Plant Breeding, Junagadh Agricultural University, India.
H. G. Vora
Department of Genetics and Plant Breeding, Junagadh Agricultural University, India.
J. J. Bhalodiya
Department of Genetics and Plant Breeding, Junagadh Agricultural University, India.
*Author to whom correspondence should be addressed.
Abstract
The present study evaluated the impact of gamma irradiation on germination and seedling growth parameters in two sesame (Sesamum indicum L.) varieties, G. Til 2 and G. Til 6. Seeds were exposed to gamma-ray doses ranging from 250 to 550 Gy, with untreated seeds serving as control. Results showed a progressive decline in germination percentage, root length, shoot length, seedling fresh weight, seedling dry weight, and vigour indices as radiation dose increased. Among all treatments, 250 Gy exhibited the least adverse effects and least reduction in germination and stronger seedling growth in both varieties. The values recorded for all traits in the treated populations remained consistently lower than those of the untreated control.Higher doses, particularly 500 and 550 Gy, significantly reduced all growth traits, indicating severe physiological damage. The findings suggest that 250 Gy is the most suitable dose for inducing genetic variability while preserving seedling viability in sesame.
Keywords: Mutation, gamma rays, sesame, variability