Bioprospecting of Indigenous Biosurfactant Bacteria in Cassava Press Water and Garage Soils and Identification of Strains of the Genus Bacillus Sp. by Sanger Sequencing
Véronique Junior NGOMA TSATY
*
Laboratoire des Procédés Industriels de Synthèse, de l’ Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), Yamoussoukro, Côte d’Ivoire and Unité de Chimie du Végétal et de la vie (UC2V), Faculté de Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo.
Gouessé Henri BRITON BI
Laboratoire des Procédés Industriels de Synthèse, de l’ Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), Yamoussoukro, Côte d’Ivoire
Kiélé Molingo MBEMBA
Unité de Chimie du Végétal et de la vie (UC2V), Faculté de Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo.
Ahou Cinthia Inès YEBOUET
Laboratoire de Microbiologie, Biotechnologie et Bio-informatique (LaMBB), Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), Yamoussoukro, Côte d’Ivoire.
Judicael Gabriel OKENI
Unité de Chimie du Végétal et de la vie (UC2V), Faculté de Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo.
Jeansval Rossam NGOULOU
Unité de Chimie du Végétal et de la vie (UC2V), Faculté de Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo.
Claude Aimé LITEBE
Unité de Chimie du Végétal et de la vie (UC2V), Faculté de Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo.
Kouassi Benjamin YAO
Laboratoire des Procédés Industriels de Synthèse, de l’ Environnement et des Energies Nouvelles (LAPISEN), Institut National Polytechnique Félix Houphouët-Boigny (INP-HB), Yamoussoukro, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Soil pollution by hydrocarbons is a major environmental problem, particularly in oil-producing countries that are developing. The objective of this work is to carry out the bioprospecting of cassava press water and garage soil substrates with bioremediating power. Thus, apart from the physic-chemical characterization of cassava press water and garage soil substrates, the contents of trace elements such as sodium (Na), potassium (K), calcium (Ca), phosphorus (P), and magnesium (Mg) were evaluated. These results showed that organic matter (22%) is abundant, moisture (1.05%) is low, and both substrates had an acidic pH (5-6) favorable to the formation of organic acids. In addition, the petroleum hydrocarbon content (TPH = 8.67%) showed a selectivity of microorganisms to grow in polluted soils. The NT2 and VJ5 strains, isolated from cassava press water and garage soil, respectively, exhibited similar optical density values (0.20) during the tolerance test with hydrocarbons. Finally, the NT2 strain is a Gram-positive bacterium with emulsifying activity (24,67) and its identification by Sanger sequencing revealed that it is the genus Bacillus sp. Cassava press water can be an interesting matrix to be used for the bioremediation of environments polluted by hydrocarbons.
Keywords: Substrate, indigenous bacteria, bacillus, sanger sequencing