Histological Effect of Static Magnetic Fields on Testis and Epididymis in Male Mice
Nedhal I. Lateff
Department of Biology, College of Education for Women, University of Anbar, Anbar, Iraq.
Abid A. Thaker
Department of Biology, College of Science, University of Anbar, Anbar, Iraq.
Mehdi S. Shallal
Anatomy Division, College of Medicine, University of Anbar, Anbar, Iraq.
Emad M. Salah *
Department of Applied Geology, College of Science, University of Anbar, Anbar, Iraq.
*Author to whom correspondence should be addressed.
Abstract
Background: Considerable attention is focused on effects of static magnetic field (SMF) and its increasing use in everyday life. Appliances and various equipments are sources of static magnetic fields with awide – range of technical characteristics.
Objective: In this study we investigated the effect of (SMF) (750 G, 1500 G and 3000 G) on testis and epididymis duct in mice.
Materials and Methods: Fifty BALB\C mice were selected and divided in to four groups (Control and 3 experimental), while control was not exposed to SMF, the experimental group was exposed to SMF (750 G, 1500 G and 3000 G) for 30 days. At the end of 30 days, the mice were weighted sacrificed dissected, after then the testis and epididymis were weighted separately and samples from testis and epididymis duct in all groups were taken and processed for light microscopic studies. Fifty microscopic fields from each group were randomly selected. The diameters and the height of epithelial cells of seminiferous tubules and epididymis duct in 4 group were measured and compared using statistical methods.
Results: The data showed that the weight of testiclesin SMF groups (750 G and 1500 G) significantly reduced compared to the control group (P< 0.05) and the weight of epididymis insignificantly reduced. The weight of testicles and epididymisin the experimental group (3000 G) insignificantly increased compared to control group (P< 0.05). The diameter of epithelial cell of seminiferous tubules and ductus epididymis in experimental group (750 G and 1500 G) significantly decreased compared to the control group (P< 0.05) and insignificantly increased in experimental group (3000 G). The height of epithelial cell of seminiferous tubules and ductus epididymis in the experimental groups (750 G and 1500 G) significantly reduced and in the group (3000 G) significantly increased, compared to control group (P< 0.05).
Conclusion: It could be concluded that the exposure to SMF leads to detrimental effects on male reproductive system in mice as seen by adecrease in diameter of reproductive ducts, the height of epithelial cells and weight of testis and epididymis.
Keywords: Static magnetic field, testis, epididymis, reproductive system, mice, histology.