First Report of Wound Infection with Bacillus pumilus in Iran
Hossein Azizi
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Farnaz Kheirandish
Department of Parasitology and Mycology, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Fatemeh Saleh *
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Mehdi Moazzami Gudarzi
Departement of Microbiology, Faculty of Paramedical Science, Islamic Azad University, Borujerd Branch, Borujerd, Iran.
*Author to whom correspondence should be addressed.
Abstract
Aims: Bacillus pumilus is a spore-forming bacillus that causes infections which has been rarely reported among humans. This research was the first report of isolating B. pumilus from bed sore in a patient who was a rancher in Iran.
Presentation of Case: In October 2012 a man with an injured livestock in the back area with fever, pain, bleeding, inflammation, accompanied by black necrosis tissue was hospitalized. According to the patient statements ulcer was due to contamination by animal. After the wound biopsy, the infectious specimen was cultured on the blood agar culture medium and then several biochemical tests were performed in order to identify. Polymerase chain reaction (PCR) was used for determining molecular identity.
Discussion and Conclusion: PCR was used for molecular identification. Result of sequencing and blast showed 99% homology with B. pumilus According to physician orders, patient was treated with clindamycin and ceftriaxone. Results of the present research showed that Bacillus B. pumilus in addition to the gastrointestinal and skin infections in humans can be a factor in bed sore disease.
Keywords: Bacillus pumilus, PCR, bedsore, wound Infection.