Phytochemical and Antioxidant Evaluation of Moullava spicata (Dalzell) Nicolson Leaf Extract
K. Lohith
Department of Biochemistry, Mangalore University, Madikeri, Karnataka, India.
R. Vijay
Central Research Laboratory, A B Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore, Karnataka, India.
K. C. Pushpalatha *
Department of Biochemistry, Mangalore University, Madikeri, Karnataka, India.
Chandrashekara G. Joshi
Department of Biochemistry, Mangalore University, Madikeri, Karnataka, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Moullava spicata (Dalzell) Nicolson is an endemic medicinal plant and its leaves are used to treat many diseases.
Aims: The aim of the study was to evaluate the phytochemical, antifungal and antioxidant status of Moullava spicata leaf extract.
Methods and Materials: The total phenolic content of leaf was measured by Folin-Ciolteau’s assay. Antioxidant activity of the plant was detected by using in-vitro 2,2- diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS), Phosphomolybdenum reduction and Ferric reducing antioxidant power (FRAP) assay. Antifungal activity was detected by Agar cup diffusion method.
Result: The phytochemical analysis showed that extracts of M. spicata contained pharmacologically important phytochemicals. Acetone extract showed higher total antioxidants activity and reducing power. However DPPH and ABTS scavenging activity were higher in methanol and acetone extracts respectively.
Conclusion: Present study showed pharmacologically significant phytochemical and high antioxidant status on leaf.
Keywords: Moullava spicata, phytochemical, antioxidant.