Phenotypic and Molecular Detection of Extended Spectrum β-Lactamase in Escherichia coli from Patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria
A. Bassey
Laboratory Department, Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria.
Y. B. Ngwai *
Department of Microbiology, Nasarawa State University, P.M.B. 1022, Keffi, Nigeria.
B. E. Bassey
World Health Organization Nigeria Country Office, UN House, Plot 617/618, Diplomatic Drive, Central Business District, P.M.B. 2861, Garki, Abuja, Nigeria.
I. H. Nkene
Department of Microbiology, Nasarawa State University, P.M.B. 1022, Keffi, Nigeria.
R. H. Abimiku
Department of Microbiology, Nasarawa State University, P.M.B. 1022, Keffi, Nigeria.
S. K. Parom
Department of Pharmaceutics and Pharmaceutical Microbiology, Kaduna State University, Kaduna, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: The study was conducted to determine the presence of extended spectrum beta-lactamase (ESBL) among Escherichia coli isolates recovered from the urine of patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria using a phenotypic and molecular method.
Methodology: Forty five (45) confirmed E. coli recovered from urine of patients with suspected UTIs were obtained; Phenotypic detection of ESBL was done on isolates resistant to cefotaxime and ceftazidime by the double disc synergy test method. Molecular detection of ESBL genes in phenotypically confirmed ESBL producers was done using Polymerase Chain Reaction.
Results: Of the 40 cefotaxime and ceftazidime-resistant isolates tested, 12 (30.0%) were phenotypically confirmed as ESBL producers. ESBL genes were detected in the order (blaTEM: blaSHV: blaCTX-M): 75.0%: 58.3%: 25.0%. Some (16.7%) of the ESBL isolates harboured both blaTEM and blaSHV; and some harboured other combinations of the ESBL namely blaTEM/blaCTX-M (8.3%), blaSHV/blaCTX-M (8.3%) and blaTEM/blaSHV/blaCTX-M (8.3%).
Conclusion: Many of the E. coli isolates were ESBL producers; and they harboured blaTEM genes most frequently. Further studies on molecular diversity of the ESBL producing E. coli from urine in the study location should be carried out.
Keywords: Escherichia coli, beta-lactamases, urine