Annual Research & Review in Biology

This paper aims to update recent findings related to the potential factors affecting the quality of buck semen during cryopreservation process. This paper describes cryopreservation of buck semen and it identifies different factors limiting the successful use of this technique in goats. Recently, several assisted reproductive technologies have been developed to control and to improve reproductive characteristics and productivity in goats. Semen cryopreservation is one of the most commonly used assisted reproductive technologies. This reproductive method allows an accelerated production of genetically valuable offspring. It is affected by various factors limiting its successful application. The handling practices prior to freezing, the composition of extenders, temperature and the length of storage have considerable effects on reproductive outcomes reducing the quantity and quality of spermatozoa. Furthermore, extensive investigations are needed to clearly assess the mechanisms between the temperature, and generation of reactive oxygen species during cryopreservation.