Levels of Interleukin‐18 in Saliva and Gingival Crevicular Fluid in Patients with Chronic Periodontitis and Healthy Subjects
Zeinab Rezaei Esfahrood
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Davood Zare
Department of Periodontics, Faculty of Dentistry, Shahid Sadughi University of Medical Science, Yazd, Iran.
Javad Zavar Reza
Department of Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Farzaneh Rahmanian *
Faculty of Dentistry, Shahid Sadughi University of Medical Science, Yazd, Iran.
*Author to whom correspondence should be addressed.
Abstract
Aims: Cytokines play a key role in the initiation and progression of chronic periodontitis. This study aimed to measure and compare the levels of Interleukin (IL)-18 in both saliva and gingival cervicular fluid (GCF) of patients with chronic periodontitis and healthy controls.
Study Design: In this descriptive study we assessed the levels of IL-18 in unstimulated whole saliva and GCF samples among patients with chronic periodontitis and individuals with healthy periodontium.
Place and Duration of Study: Department of periodontology and Department of biochemistry, Services Institute of Shahid Sadughi University of Medical Science, between May 2015 and Jan 2016.
Methodology: Thirty two subjects were divided into two groups; 16 individuals with healthy periodontium and 16 patients with chronic periodontitis. GCF and saliva samples were obtained. The concentrations of IL-18 in GCF and saliva were determined using ELISAs.
Results: There was no significant difference between the levels of IL-18 in saliva and GCF samples among the study groups (Respectively P= 0.44 and P= 0.38). Also, significantly different was not observed while comparing the IL-18 level of saliva with the IL-18 level of GCF in both healthy subjects (P= 0.8) and patients with chronic periodontitis (P= 0.61).
Conclusion: Similar levels of IL-18 among study groups suggested that levels of IL-18 in saliva and GCF cannot be used as a predictable biomarker for early diagnosis of periodontal disease.
Keywords: Gingival crevicular fluid, interleukin‐18, periodontitis, saliva.