Effects of Vitamin E Administration on Certain Biochemical and Antioxidants Indices in Some Rat Tissues Treated with Aluminium Chloride
O. T. Adedosu *
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Nigeria.
G. E. Adeleke
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Nigeria.
T. A. Alao
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Nigeria.
N. E. Ojugo
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Nigeria.
O. E. Akinsoji
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomoso, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: It has been shown that aluminium and its compounds accumulate in some tissues causing metabolic dysfunctions. This study investigated the effects of vitamin E administration on certain biochemical and antioxidants indices in rat tissues treated with aluminium chloride (AlCl3).
Study Design: Twenty four male Wistar rats with average weight of 160 g were randomly selected into four groups and treated as follows; group A(distilled water), group B (vitamin E only,100 mg/kg. bw/day), group C (AlCl3 only,40 mg/kg. bw/day) and group D (vitamin E and AlCl3), all administered orally in 0.2 ml solution for 21 days.
Methodology: Biochemical and antioxidant indices were evaluated using standard methods.
Results: Results showed that treatment with AlCl3 only (group C) caused significant (P=.05) decreases in Red Blood Cell (WBC) count, Packed Cell Volume (PCV), serum total protein and High Density Lipoprotein-Cholesterol ( HDL-C) with increases in White Blood Cell (WBC) count, triglyceride and total cholesterol. It also elicits significant (P=.05) increases in some liver and kidney indices such as Alanine Aminotransferase (ALT), Gamma Glutamyl Transferase (GGT), alkaline phosphatase (ALP) activities, serum urea and creatinine concentrations. Analysis of antioxidant indices of the liver, kidney, brain and testis also revealed decreases in activities of Superoxide Dismutase (SOD), Catalase (CAT) and Reduced Glutathione (GSH) concentration with corresponding significant (P=.05) increases in Malondialdehyde (MDA) level by AlCl3 administration. In contrast, treatment with vitamin E and AlCl3 (group D) showed improved in metabolic alterations observed in AlCl3 intoxication as these indices were reversed with greater effects observed in group B.
Conclusion: Results obtained are indication of antioxidative and tissue protective effects of vitamin E against dyslipidemia and heamatological disorder induced by (AlCl3).
Keywords: Antioxidative, vitamin E, aluminium chloride, dyslipidemia, heamatological disorder