Effect of Temperature on Production of Endoglucanase and Xylanase from Some Green Manure Fungal Decomposers
Ravindra Kumar *
ICAR-Indian Agricultural Research Institute, Regional Station, Karnal-132 001 (Haryana), India.
Asha Sinha
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005 (UP), India.
Manisha Srivastava
Sri A.K. P.G. College, Varanasi-221005 (UP), India.
Seweta Srivastava
School of Agriculture, Lovely Professional University, Phagwara-144 411 (Punjab), India.
Anirudha Chattopadhyay
Department of Plant Pathology, C.P. College of Agriculture, S.D. Agricultural University, S.K. Nagar-385506, Gujarat, India.
*Author to whom correspondence should be addressed.
Abstract
Among the various microorganisms reported for their capabilities of cellulases and xylanases production, fungi are the most potent producers. This study reports the production of xylanolytic and cellulolytic enzymes by potential decomposing fungal isolates using a cheap medium containing green manure substrate and chemically defined basal medium under solid-state culture. A number of decomposing fungi were screened for cellulolytic and xylanolytic potential. The enzyme production with purified carboxy methyl cellulose (CMC) and birch wood xylan was studied and found to be promising. Aspergillus niger produced the highest amount of endoglucanase and xylanase viz., 3.635 U mg-1 protein min-1 and 2498.20 U mg-1 protein min-1, respectively at 25ºC. Growth and enzyme production was affected by the alterations in temperature. Highest production of endoglucanse and xylanase were noted at 25ºC and 30ºC.
Keywords: Endoglucanase, fungal decomposers, Sesbania aculeata L. and xylanase