Plantlet Regeneration from Unfertilized Ovule of Mandarin (Citrus reticulata)
Label Kawtar
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco and Laboratory of Biodiversity and Natural Resources, Faculty of Science Kenitra, Tofail University, Ibn, Morocco
Handaji Najat *
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco.
Brhadda Najiba
Laboratory of Biodiversity and Natural Resources, Faculty of Science Kenitra, Tofail University, Ibn, Morocco.
Arsalane Najat
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco.
Gmira Najib
Laboratory of Biodiversity and Natural Resources, Faculty of Science Kenitra, Tofail University, Ibn, Morocco.
Aderdour Tarik
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco and Laboratory of Biodiversity and Natural Resources, Faculty of Science Kenitra, Tofail University, Ibn, Morocco
Karim Mahmoudi
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco and Laboratory of Biodiversity and Natural Resources, Faculty of Science Kenitra, Tofail University, Ibn, Morocco
Benyahia Hamid
Laboratory for the Improvement and Conservation of Plant Genetic Resources, National Institute of Agronomic Research (INRA), Kenitra, Morocco.
*Author to whom correspondence should be addressed.
Abstract
The genus Citrus contains numerous fresh and processed fruit cultivars that are economically important worldwide, many genotypes are amenable to somatic embryogenesis, which became a key regeneration pathway in many experimental approaches of cultivar improvement. in this objective We aime at studying the effects of various culture media on the induction and the development of citrus somatic embryos.Callus cultures were initiated from the infertlized ovules of six varieties of mandarin (Anana, Lee, Murcott, Ortanique, Temple, and Wilking) within 3 media: MT (Murashig and Tuker, 1969) without hormones, MT + 1 mg/l BAP, MT + 1 mg/l Kinetin, the experiments show a highly significant effect (P < 0.001) of the culture media and genotype. No reactivity was observed on the MT environment in the absence of growth regulator, while the culture media MT in addition to 1 mg/l BAP gave the best results of induction of embryogenic callus induction. The induction of somatic embryogenesis was obtained on MT media without hormones. For the plantlets regeneration the favorable media was MT without hormones or added to ANA and active coal.
Keywords: Citrus, mandarin, somaclonal variation, callus, somatic embryogenesis, regeneration of seedlings