In vivo Antithrombotic Activity of Ethanolic Extract from Ocimum basilicum L.
Abudukadeer Kuerban *
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia.
Yaaser Q. Almulaiky
Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia and Department of Chemistry, Faculty of Applied Science, Taiz University, Yemen and Center of University of Jeddah for Science and Medical Research, Jeddah, Kingdom of Saudi Arabia.
Ryan A. Sheikh
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia.
Saeed Ahmed Alzahrani
Medical College of Al-Baha University, Al-Baha, Kingdom of Saudi Arabia.
Hasan A. Al-Talhi
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia.
Md. Nasrullah
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia.
Said Salama Moselhy
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia and Bioactive Natural Products Research Group, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia and Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Khalid Omar Abulnaja
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia and Bioactive Natural Products Research Group, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
Aims: In this study, the antithrombotic activity of ethanolic extract from Ocimum basilicum L. was investigated on mice. The active component of Ocimum basilicum L. was extracted in 40% ethanol by Soxhlet extractor.
Place and Duration of Study: Department of Pharmacology, Faculty of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, China, between February, 2009 and July, 2009.
Methodology: The crude extract was gavaged to mice in different concentration (100mg/ml, 200 mg/ml, and 400 mg/ml), for 21 days, Salvia Tablets were used as a positive control. On the final day, 50 mg/kg carrageenan was injected intraperitoneally to induce blood coagulation. Fibrin degradation products (FDPs) were assayed.
Results: Results showed that the content of mice FDPs significantly（P<0. 05）increased in the 200 mg/ml dose group and 400mg/ml dose group compared to the control group. This was dose-dependent.
Conclusion: The ethanolic extract of Ocimum basilicum L can activate the fibrinolytic system so as to stimulate Plasminogen enzyme into fibrinolytic enzyme to show antithrombotic activity.
Keywords: Ocimum basilicum L, Plasminogen, fibrinolytic enzyme, FDPs