In vitro Antiglycation, Antioxidant Properties of Coleus forskohlii ''Balady'' Leaves and Stem and their Antioxidant Enzyme Activities
Yaaser Q. Almulaiky
Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia and Chemistry Department, Faculty of Applied Science, Taiz University, Yemen and Center of University of Jeddah for Science and Medical Research, Kingdom of Saudi Arabia.
Abudukadeer Kuerban *
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Faisal Aqlan
Center of University of Jeddah for Science and Medical Research, Kingdom of Saudi Arabia and Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia.
Saeed Ahmed Alzahrani
Medical College of Al-Baha University, Al Baha, Kingdom of Saudi Arabia.
Mohammed N. Baeshen
Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia.
Mohamed Afifi
Center of University of Jeddah for Science and Medical Research, Kingdom of Saudi Arabia and Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Egypt and Department of Biological Science, Faculty of Science, University of Jeddah, Kingdom of Saudi Arabia.
Ammar Alfarga
Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia.
Waleed Al-Shawafi
Chemistry Department, Faculty of Applied Science, Taiz University, Yemen and Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Mohammed Alkhaled
Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia and Department of Biological Science, Faculty of Science, University of Jeddah, Kingdom of Saudi Arabia.
*Author to whom correspondence should be addressed.
Abstract
Aims: In this study, the antiglycation and antioxidant potency of methanol (80%) extracts, as well as the antioxidant enzyme activities from both leaves and stem of Coleus forskohlii “balady”, were analyzed.
Place and Duration of Study: Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, between February 2017 and July 2017.
Methodology: Antiglycation activity was evaluated by BSA-glucose glycation assay. The total phenolics, flavonoids, chlorophyll, carotenoid, lycopene content were estimated, and antioxidant activity was tested by the capacity to reduce ABTS reducing power assay, DPPH radical-scavenging activity assay, FRAP assay. Enzyme activities from both leaves and stem of Coleus forskohlii were tested for polyphenol oxidase, peroxidase, and catalase.
Results: Results indicated that methanolic extracts from leaves exhibited strong antiglycation and antioxidant activities than from stem. Among the enzymatic antioxidant properties, except for the activity of peroxidase, the activities of catalase and polyphenol oxidase were significantly higher (P < 0.05) in leaves than in stem.
Conclusion: It can be concluded that the leaves contained significantly rich sources of both enzymatic and non-enzymatic antioxidants can be used as a natural antioxidants.
Keywords: Methacrylamido phenylboronic acid, antioxidant, antiglycation, enzyme, coleus forskohlii ''Balady'', stem, leaves.