Novel Cathelicidin Antimicrobial Peptides from Paa robertingeri
Qinghua Luo
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, 550001, China.
Huaiqing Deng
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, 550001, China.
Mengguang Yin
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, 550001, China.
Chen Chen
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, 550001, China.
Jiang Zhou *
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, 550001, China.
*Author to whom correspondence should be addressed.
Abstract
This study aimed to describe two cathelicidins (cathelicidin-PR1 and cathelicidin-PR2) from the skin of Paa robertingeri (Anura: Ranidae). The deduced mature peptides cathelicidin-PR1 and cathelicidin-PR2 were composed of 29 and 25 residues, respectively. Cathelicidin - PR1 has higher antimicrobial activity it could kill Gram-positive and Gram-negative bacteria and even some fungal species. Cathelicidin-PR1 exhibited more effective than AMP in antimicrobial activity against Pseydomonas maltophilia clinical strain. On the contrary, cathelicidin-PR2 had very weak antimicrobial activity. Furthermore, cathelicidin-PR1 and cathelicidin-PR2 exhibited very low hemolytic activity against human erythrocytes and little hemagglutinating activity. The results suggested that the cathelicidin-PR1 might serve as a template for developing novel antibiotics.
Keywords: Antimicrobial activity, antimicrobial peptide, hemagglutinating activity, hemolytic activity, Paa robertingeri.