Improved Decipherment of the Protein Database of Human Proteins in the PDMD (Protein-Direct-Microsequencing-Deciphering) Method
Kou Hayakawa *
Department of Endoclinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
*Author to whom correspondence should be addressed.
Abstract
Human proteins seem to be processed by Human serum biotinidase, and Human excreted proteins seem to be handled with by Human serum biotinidase and Human Chymotrypsin A. Therefore, we must improve PDMD method by using these new findings. Protein determination is performed by the highly sensitive HPLC-SEC-photometric method at UV 210 nm; i.e., c.a. 200-fold sensitive than Lowry’s method. Human proteins are found to be not metabolized at membrane inserted portions. Membrane and Hydrophobic proteins of Humans are defined as the precipitable proteins at 100,000 x g for 90 min at 4 C, and have hydrophobicities larger than 0.515.
Keywords: Microsequencing, Edman degradation, HPLC-with photometric detection, proteomics, protein determination