Investigation of the Presence of Norovirus (GI, GII) and Hepatitis A virus with Real-time RT-PCR test in Milk Cans of Small Family Enterprises that Selling Raw Milk
Mehmet Recai YILMAZ
Department of Virology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Mehmet KALE
*
Department of Virology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
*Author to whom correspondence should be addressed.
Abstract
In this study, the presence of Norovirus GI, NoV GII and Hepatitis A Virus was investigated in milk cans where the milk of dairy cows aged 24 months and older were collected from small family enterprises selling raw milk in Burdur-Merkez. In the Real-Time RT-PCR analysis performed to determine the presence of NoV and HAV in milk samples taken from 100 small family enterprises selling raw milk in Burdur-Centre; 16% NoV GI, 12% NoV GII, 8% HAV positivity was detected. In the results obtained as co-infection; 4% NoV GI + NoV GII and 8% NoV GI + NoV GII + HAV were determined. The mean SCC in the milk obtained in the study was determined as 339.020+249.230.9. In this study, the mean SCCs of NoV GI, NoV GII and HAV positive samples were statistically lower than the mean SCCs of negative samples (p<0,05). The mean SCC of NoV GI positive samples was 225.375+60.018,9 (p=0,005), the mean SCC of NoV GII positive samples was 251.166,7+42.994,9 (p=0,01) and the mean SCC of HAV positive samples was 248.750+49.969,1 (p=0,04). It was concluded that the presence of NoV GI, NoV GII and HAV in milk did not affect SCC and could not be used as an indicator of viral presence in milk.
Keywords: Hepatitis A virus, Norovirus, milk can, raw milk, real-time RT-PCR