Biological Activities of a Recipe Consisting of a Mixture of Two Plants Fron the Ivorian Pharmacopoeia: Nephrolepis bisserata and Clerodendrum splendens (NC)
Tidou Sylvie Noëlle
UFR Bioscience, Laboratories of Plant Biology of University of Felix Houphouet-Boigny, Ivory Coast.
Kouadio Kouakou John *
UFR Bioscience, Laboratories of Biology and health of University of Felix Houphouet-Boigny, Ivory Coast.
Bohoussou Crystel Natacha
UFR Bioscience, Laboratories of Plant Biology of University of Felix Houphouet-Boigny, Ivory Coast.
Zirihi Guède Noël
UFR Bioscience, Laboratories of Plant Biology of University of Felix Houphouet-Boigny, Ivory Coast.
*Author to whom correspondence should be addressed.
Abstract
Natural plant extracts contain a variety of biologically active molecules. In this context, we attempted to determine and evaluate the biological activity of mixture of Nephrolepis bisserata and Clerodendrum splendens (NC). Qualitative analysis of these extracts by preliminary tube tests revealed the presence of phenolic compounds, tannins, flavonoids and saponins in both extracts, as well as the mixture. Antioxidant activity was established by the FRAP method and the TEAC test in vitro, the results obtained demonstrate the presence of the antioxidant active ingredients in both extracts with different proportions. The acute toxicity was done according to the OECD 423 method. The results showed that the LD50 of the mixture (NC) was greater than 2000 mg/kg of mc. As for the biological activity of the mixture (NC), it was done according to the OECD 407 method. The results showed that the daily administration for 28 days of the mixture did not disturb physiological, physical, biochemical state of the rats.
Keywords: Clerodendrum splendens, Nephrolepis bisserata, biological activity, FRAP, TEAC, toxicity